Detailed information of TA system TA160026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 20129..20654
• Replicon: plasmid unnamed1
• Accession: NZ_CP054663
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: HUR93_RS25775
• Protein ID: WP_032334755.1
• Coordinates: 20349..20654 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: HUR93_RS25770
• Protein ID: WP_032334753.1
• Coordinates: 20129..20347 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS25745 (17333)	17333..18232	-	900	Protein_16	IS110 family transposase	-
HUR93_RS25750 (18320)	18320..18667	+	348	WP_032334749.1	DUF6404 family protein	-
HUR93_RS25755 (18685)	18685..19275	-	591	WP_032334750.1	hypothetical protein	-
HUR93_RS25760 (19275)	19275..19532	-	258	WP_000371885.1	hypothetical protein	-
HUR93_RS25765 (19760)	19760..20002	+	243	Protein_20	hypothetical protein	-
HUR93_RS25770 (20129)	20129..20347	+	219	WP_032334753.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
HUR93_RS25775 (20349)	20349..20654	+	306	WP_032334755.1	type II toxin-antitoxin system toxin CcdB	Toxin
HUR93_RS25780 (20655)	20655..21461	+	807	WP_061892142.1	site-specific integrase	-
HUR93_RS25785 (22264)	22264..22454	-	191	Protein_24	hypothetical protein	-
HUR93_RS25790 (22977)	22977..23891	+	915	WP_073972117.1	transcriptional regulator TdcA	-
HUR93_RS25795 (23931)	23931..25100	-	1170	WP_000656436.1	M20 family metallopeptidase	-
HUR93_RS25800 (25116)	25116..25577	-	462	WP_237897982.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..108557	108557
	-	flank	IS/Tn	-	-	17333..18187	854

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11678.46 Da
• Isoelectric Point: 6.4663

>T160026 WP_032334755.1 NZ_CP054663:20349-20654 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRDLYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T160026 NZ_CP070281:2375903-2376181 [Escherichia albertii]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTATTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCATTAAGCGGCGATCGCGATGGCATCTGGGCAATATCTGTATCAGGCAACTGGCGAATCACATTTGAGTTCGTCAAT
GGCAATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8342.27 Da
• Isoelectric Point: 4.5203

>AT160026 WP_032334753.1 NZ_CP054663:20129-20347 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSATMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT160026 NZ_CP070281:2376181-2376465 [Escherichia albertii]
ATGAAAATGGTCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGACTGTTGACCGGAAAAGCGGCTTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCTGAG
AAAACGGTGGATGTGTCGCGGTTGCGCCGTCTGGTTACGCAGTAG