Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4738435..4738932 Replicon chromosome
Accession NZ_CP054662
Organism Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HUR93_RS23195 Protein ID WP_001173343.1
Coordinates 4738435..4738608 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HUR93_RS23200 Protein ID WP_000398619.1
Coordinates 4738636..4738932 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR93_RS23185 (4735105) 4735105..4736562 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
HUR93_RS23190 (4736799) 4736799..4738316 + 1518 WP_001295087.1 lysine--tRNA ligase -
HUR93_RS23195 (4738435) 4738435..4738608 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HUR93_RS23200 (4738636) 4738636..4738932 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HUR93_RS23205 (4739159) 4739159..4739431 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
HUR93_RS23210 (4739443) 4739443..4739673 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HUR93_RS23215 (4739854) 4739854..4741485 + 1632 WP_001216487.1 sensor histidine kinase -
HUR93_RS23220 (4741482) 4741482..4742201 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4710840..4738932 28092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T160024 WP_001173343.1 NZ_CP054662:c4738608-4738435 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T160024 NZ_CP070281:2026915-2027018 [Escherichia albertii]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAAAAC
AGGAATCGTTTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT160024 WP_000398619.1 NZ_CP054662:c4738932-4738636 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT160024 NZ_CP070281:c2027020-2026788 [Escherichia albertii]
ATAAAAAGAGACCGAAAACGATTCCTGTTTTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTACAAAATGGTCAG
TAAAAAGCGCGTCGGCCATCGTCTTAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGTGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References