Detailed information of TA system TA160020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3896459..3897296
• Replicon: chromosome
• Accession: NZ_CP054662
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: HUR93_RS19315
• Protein ID: WP_000227784.1
• Coordinates: 3896754..3897296 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: HUR93_RS19310
• Protein ID: WP_001297137.1
• Coordinates: 3896459..3896770 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS19285 (3891479)	3891479..3892426	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
HUR93_RS19290 (3892448)	3892448..3894439	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
HUR93_RS19295 (3894429)	3894429..3895043	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
HUR93_RS19300 (3895043)	3895043..3895372	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
HUR93_RS19305 (3895384)	3895384..3896274	+	891	WP_000971336.1	heme o synthase	-
HUR93_RS19310 (3896459)	3896459..3896770	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
HUR93_RS19315 (3896754)	3896754..3897296	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
HUR93_RS19320 (3897352)	3897352..3898287	-	936	WP_001368479.1	tetratricopeptide repeat protein	-
HUR93_RS19325 (3898695)	3898695..3900059	+	1365	WP_001000960.1	MFS transporter	-
HUR93_RS19330 (3900187)	3900187..3900678	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
HUR93_RS19335 (3900846)	3900846..3901757	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T160020 WP_000227784.1 NZ_CP054662:3896754-3897296 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T160020 NZ_CP070281:279141-279668 [Escherichia albertii]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTTTGCGAGCGTATATCCTTTGCCGCAACACTC
CAGAACGTCAGGTCCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCATTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCTCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCGTTGGTTGGAGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT160020 WP_001297137.1 NZ_CP054662:3896459-3896770 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT160020 NZ_CP070281:278868-279134 [Escherichia albertii]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAATCCGTTAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGTTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3