Detailed information of TA system TA160002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1544328..1544953
• Replicon: chromosome
• Accession: NZ_CP054662
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUR93_RS07490
• Protein ID: WP_000911330.1
• Coordinates: 1544555..1544953 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HUR93_RS07485
• Protein ID: WP_000450524.1
• Coordinates: 1544328..1544555 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS07460 (1540130)	1540130..1540600	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HUR93_RS07465 (1540600)	1540600..1541172	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HUR93_RS07470 (1541318)	1541318..1542196	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HUR93_RS07475 (1542213)	1542213..1543247	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
HUR93_RS07480 (1543460)	1543460..1544173	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HUR93_RS07485 (1544328)	1544328..1544555	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HUR93_RS07490 (1544555)	1544555..1544953	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUR93_RS07495 (1545100)	1545100..1545963	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
HUR93_RS07500 (1545978)	1545978..1547993	+	2016	WP_000829294.1	tRNA cytosine(34) acetyltransferase TmcA	-
HUR93_RS07505 (1548067)	1548067..1548765	+	699	WP_000679823.1	esterase	-
HUR93_RS07510 (1548875)	1548875..1549075	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T160002 WP_000911330.1 NZ_CP054662:1544555-1544953 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T160002 NZ_CP070232:1972997-1973100 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT160002 WP_000450524.1 NZ_CP054662:1544328-1544555 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT160002 NZ_CP070232:c1973138-1972867 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A829CM33