Detailed information of TA system TA160000

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1164796..1165631
• Replicon: chromosome
• Accession: NZ_CP054662
• Organism: Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A3L5HA82
• Locus tag: HUR93_RS05620
• Protein ID: WP_061090001.1
• Coordinates: 1165254..1165631 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HUR93_RS05615
• Protein ID: WP_032256355.1
• Coordinates: 1164796..1165164 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUR93_RS05580 (1160243)	1160243..1160722	+	480	WP_061892424.1	antirestriction protein	-
HUR93_RS05585 (1160738)	1160738..1161211	+	474	Protein_1091	RadC family protein	-
HUR93_RS05590 (1161283)	1161283..1161504	+	222	WP_000692303.1	DUF987 domain-containing protein	-
HUR93_RS05595 (1161527)	1161527..1162060	+	534	Protein_1093	antitoxin of toxin-antitoxin stability system	-
HUR93_RS05600 (1162144)	1162144..1162890	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
HUR93_RS05605 (1162905)	1162905..1164446	-	1542	WP_061892665.1	IS21-like element ISEc12 family transposase	-
HUR93_RS05610 (1164636)	1164636..1164746	+	111	Protein_1096	antitoxin of toxin-antitoxin stability system	-
HUR93_RS05615 (1164796)	1164796..1165164	+	369	WP_032256355.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HUR93_RS05620 (1165254)	1165254..1165631	+	378	WP_061090001.1	TA system toxin CbtA family protein	Toxin
HUR93_RS05625 (1165628)	1165628..1166116	+	489	WP_061090000.1	DUF5983 family protein	-
HUR93_RS05630 (1166134)	1166134..1166331	+	198	WP_032256361.1	DUF957 domain-containing protein	-
HUR93_RS26760 (1166416)	1166416..1167257	+	842	Protein_1101	DUF4942 domain-containing protein	-
HUR93_RS05645 (1167538)	1167538..1168074	-	537	WP_000942799.1	GspM family type II secretion system protein YghD	-
HUR93_RS05650 (1168076)	1168076..1169254	-	1179	WP_000094973.1	type II secretion system protein GspL	-
HUR93_RS05655 (1169251)	1169251..1170228	-	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14047.00 Da
• Isoelectric Point: 7.8049

>T160000 WP_061090001.1 NZ_CP054662:1165254-1165631 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T160000 NZ_CP070232:1763447-1763701 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13715.44 Da
• Isoelectric Point: 5.8808

>AT160000 WP_032256355.1 NZ_CP054662:1164796-1165164 [Escherichia coli]
VSDSLHETNYPDDHNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT160000 NZ_CP070232:1763199-1763450 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A3L5HA82