Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1007993..1008647 Replicon chromosome
Accession NZ_CP054662
Organism Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag HUR93_RS04840 Protein ID WP_000244772.1
Coordinates 1007993..1008400 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HUR93_RS04845 Protein ID WP_000354046.1
Coordinates 1008381..1008647 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR93_RS04820 (1003950) 1003950..1005683 - 1734 WP_000813210.1 single-stranded-DNA-specific exonuclease RecJ -
HUR93_RS04825 (1005689) 1005689..1006399 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HUR93_RS04830 (1006424) 1006424..1007320 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HUR93_RS04835 (1007432) 1007432..1007953 + 522 WP_001055874.1 flavodoxin FldB -
HUR93_RS04840 (1007993) 1007993..1008400 - 408 WP_000244772.1 protein YgfX Toxin
HUR93_RS04845 (1008381) 1008381..1008647 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HUR93_RS04850 (1008890) 1008890..1009870 + 981 WP_001472148.1 tRNA-modifying protein YgfZ -
HUR93_RS04855 (1009947) 1009947..1010606 - 660 WP_000250274.1 hemolysin III family protein -
HUR93_RS04860 (1010770) 1010770..1011081 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
HUR93_RS04865 (1011126) 1011126..1012559 + 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
HUR93_RS04870 (1012616) 1012616..1013359 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T159999 WP_000244772.1 NZ_CP054662:c1008400-1007993 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T159999 NZ_CP070232:1763199-1763450 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT159999 WP_000354046.1 NZ_CP054662:c1008647-1008381 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT159999 NZ_CP070232:1763447-1763701 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References