Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 354855..355486 Replicon chromosome
Accession NZ_CP054662
Organism Escherichia coli strain IGC_RcoliRes_1.1

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HUR93_RS01605 Protein ID WP_001259388.1
Coordinates 354855..355130 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HUR93_RS01610 Protein ID WP_000593555.1
Coordinates 355127..355486 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUR93_RS01590 (349859) 349859..350926 + 1068 WP_237897951.1 HlyD family secretion protein -
HUR93_RS01595 (350923) 350923..353658 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HUR93_RS01600 (353658) 353658..354782 + 1125 WP_001314210.1 ABC transporter permease -
HUR93_RS01605 (354855) 354855..355130 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HUR93_RS01610 (355127) 355127..355486 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HUR93_RS01615 (355606) 355606..356007 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HUR93_RS01620 (356013) 356013..356819 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HUR93_RS01625 (356816) 356816..357580 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HUR93_RS01630 (357580) 357580..358413 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HUR93_RS01635 (358410) 358410..359354 - 945 WP_061892553.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T159996 WP_001259388.1 NZ_CP054662:354855-355130 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T159996 NZ_CP070232:717467-717763 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT159996 WP_000593555.1 NZ_CP054662:355127-355486 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT159996 NZ_CP070232:717765-718160 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References