Detailed information of TA system TA159992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 786981..787488
• Replicon: chromosome
• Accession: NZ_CP054626
• Organism: Cupriavidus gilardii strain FDAARGOS_639

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: FOB47_RS13710
• Protein ID: WP_174782430.1
• Coordinates: 787318..787488 (-)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: FOB47_RS13705
• Protein ID: WP_174782429.1
• Coordinates: 786981..787310 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOB47_RS13685	783154..783585	-	432	WP_174781317.1	hypothetical protein	-
FOB47_RS13690	783970..785298	+	1329	WP_174781318.1	TrkH family potassium uptake protein	-
FOB47_RS13695	785288..785986	+	699	WP_170265835.1	TrkA family potassium uptake protein	-
FOB47_RS13700	786113..786973	-	861	WP_174781319.1	aminoglycoside 6-adenylyltransferase	-
FOB47_RS13705	786981..787310	-	330	WP_174782429.1	BrnA antitoxin family protein	Antitoxin
FOB47_RS13710	787318..787488	-	171	WP_174782430.1	BrnT family toxin	Toxin
FOB47_RS13715	787632..788813	-	1182	WP_174781320.1	benzoate/H(+) symporter BenE family transporter	-
FOB47_RS13720	789290..790495	+	1206	WP_174782431.1	NAD(P)/FAD-dependent oxidoreductase	-
FOB47_RS13725	790550..791257	+	708	WP_174781321.1	two-component system response regulator CreB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	776338..917425	141087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-48)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (19-99)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6651.61 Da
• Isoelectric Point: 9.0343

>T159992 WP_174782430.1 NZ_CP054626:c787488-787318 [Cupriavidus gilardii]
ILIDERRQYGETRYIAYGPIGTRLQCLVFTIRGDTLRAISLRKANFREVRDHEQEI

Nucleotide

• Length: 171 bp

>T159992 NZ_CP070231:c34684-34424 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11943.71 Da
• Isoelectric Point: 10.2632

>AT159992 WP_174782429.1 NZ_CP054626:c787310-786981 [Cupriavidus gilardii]
MPAADENAAIARGIAGDPDAVELTTEQIKRMRPAREALAEVLGEKNVEALIKRRGRPALPAAERKVSQTLRLDPDVLQAF
KATGDGWQTRINDALRAYAKSHRMLPRGC

Nucleotide

• Length: 330 bp

>AT159992 NZ_CP070231:c34434-34183 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA