Detailed information of TA system TA159988

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3561509..3562730
• Replicon: chromosome
• Accession: NZ_CP054623
• Organism: Pseudomonas aeruginosa strain DL201330

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: HUF09_RS16545
• Protein ID: WP_003110488.1
• Coordinates: 3562152..3562730 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUF09_RS16540
• Protein ID: WP_003088112.1
• Coordinates: 3561509..3562063 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF09_RS16515	3557077..3557628	+	552	WP_003450159.1	XRE family transcriptional regulator	-
HUF09_RS16520	3557625..3558023	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUF09_RS16525	3558036..3558359	-	324	WP_015648646.1	multidrug efflux SMR transporter	-
HUF09_RS16530	3558690..3559151	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUF09_RS16535	3559156..3561351	+	2196	WP_003122807.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUF09_RS16540	3561509..3562063	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HUF09_RS16545	3562152..3562730	+	579	WP_003110488.1	HD domain-containing protein	Toxin
HUF09_RS16550	3562839..3564026	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUF09_RS16555	3564016..3566187	-	2172	WP_025297367.1	type I secretion system permease/ATPase	-
HUF09_RS16560	3566177..3567454	-	1278	WP_198580692.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T159988 WP_003110488.1 NZ_CP054623:3562152-3562730 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T159988 NZ_CP070230:c16293-15988 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT159988 WP_003088112.1 NZ_CP054623:3561509-3562063 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT159988 NZ_CP070230:c16513-16295 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCTGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4