Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 1113495..1114916 Replicon chromosome
Accession NZ_CP054623
Organism Pseudomonas aeruginosa strain DL201330

Toxin (Protein)


Gene name rnlA Uniprot ID -
Locus tag HUF09_RS05285 Protein ID WP_025297643.1
Coordinates 1113843..1114916 (-) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID -
Locus tag HUF09_RS05280 Protein ID WP_158025315.1
Coordinates 1113495..1113788 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUF09_RS05235 1108598..1108810 + 213 WP_023128602.1 hypothetical protein -
HUF09_RS05240 1108901..1109356 - 456 WP_025297646.1 helix-turn-helix domain-containing protein -
HUF09_RS05245 1109521..1109916 + 396 WP_023128603.1 hypothetical protein -
HUF09_RS05250 1109959..1110429 + 471 WP_023128604.1 hypothetical protein -
HUF09_RS05255 1110432..1110815 + 384 WP_025297645.1 hypothetical protein -
HUF09_RS05260 1110897..1111475 + 579 WP_025297644.1 hypothetical protein -
HUF09_RS05265 1111806..1112170 + 365 Protein_1041 DNA binding protein -
HUF09_RS05270 1112394..1112588 - 195 WP_023128607.1 OprD family outer membrane porin -
HUF09_RS05275 1112587..1113282 + 696 WP_196747054.1 transporter substrate-binding domain-containing protein -
HUF09_RS05280 1113495..1113788 - 294 WP_158025315.1 type II toxin-antitoxin system RnlB family antitoxin Antitoxin
HUF09_RS05285 1113843..1114916 - 1074 WP_025297643.1 type II toxin-antitoxin system RnlA family toxin Toxin
HUF09_RS05290 1115631..1115885 + 255 WP_023128610.1 hypothetical protein -
HUF09_RS05295 1116183..1116479 + 297 WP_023128612.1 hypothetical protein -
HUF09_RS05300 1116532..1118115 - 1584 WP_025297642.1 hypothetical protein -
HUF09_RS05305 1118395..1118823 + 429 WP_025297641.1 S24 family peptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1099668..1121812 22144


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal

(6-87)

Antitoxin

Antitoxin RnlB/LsoB

(2-96)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40381.89 Da        Isoelectric Point: 6.1830

>T159985 WP_025297643.1 NZ_CP054623:c1114916-1113843 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Download         Length: 1074 bp

>T159985 NZ_CP070229:4101769-4102080 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.38 Da        Isoelectric Point: 4.5163

>AT159985 WP_158025315.1 NZ_CP054623:c1113788-1113495 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Download         Length: 294 bp

>AT159985 NZ_CP070229:4102077-4102496 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References