159976

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 5264197..5264832 Replicon chromosome
Accession NZ_CP054614
Organism Paenibacillus barcinonensis strain KACC11450

Toxin (Protein)


Gene name mazF Uniprot ID W4B4V0
Locus tag HUB98_RS23540 Protein ID WP_024630714.1
Coordinates 5264482..5264832 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2W6NI96
Locus tag HUB98_RS23535 Protein ID WP_024630715.1
Coordinates 5264197..5264478 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUB98_RS23515 5259900..5260466 - 567 WP_167433857.1 DUF3169 family protein -
HUB98_RS23520 5260450..5260677 - 228 WP_110899602.1 helix-turn-helix transcriptional regulator -
HUB98_RS23525 5261157..5262374 + 1218 WP_110899601.1 outer membrane lipoprotein-sorting protein -
HUB98_RS23530 5262750..5263937 + 1188 WP_110899576.1 alanine racemase -
HUB98_RS23535 5264197..5264478 + 282 WP_024630715.1 ribbon-helix-helix protein, CopG family Antitoxin
HUB98_RS23540 5264482..5264832 + 351 WP_024630714.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HUB98_RS23545 5265078..5267267 + 2190 WP_110899575.1 alpha-galactosidase -
HUB98_RS23550 5267572..5269764 + 2193 WP_174812330.1 RNA-binding transcriptional accessory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-46)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12746.71 Da        Isoelectric Point: 5.1184

>T159976 WP_024630714.1 NZ_CP054614:5264482-5264832 [Paenibacillus barcinonensis]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTCIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKLVNEALQISLGLIDF

Download         Length: 351 bp

>T159976 NZ_CP070229:c2549257-2548871 [Escherichia coli]
GTGCTTGCCCCAACTGGGCCATGCAATCATCTTCATGTGATTTGTAATGATCCTGTTTATTACCCCGTTAACGATTGTTA
TTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCCACGATCCGTCTTGCGTCTTGAATTCTGGTGATC
ATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATAATTTGGCGAGTGGATAACATGGTTAGAAAGCAG
CGATCGGGTGAGATTTCCGTTCATGATGATATGCCAGAAGCTACATTCAATAGAATTCTGGACGGTTTTGATATATCTGA
TGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTATCATCTATTGATGATGAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10723.33 Da        Isoelectric Point: 5.8649

>AT159976 WP_024630715.1 NZ_CP054614:5264197-5264478 [Paenibacillus barcinonensis]
VANLQNTKRIMISLPDYLLQEVDGIVALENSNRSELIRQAMKLYLTERKKRYIRESMQRGYMEMAKINLTMASEAFHAEE
DADSTLDRLVSGV

Download         Length: 282 bp

>AT159976 NZ_CP070229:c2549796-2549287 [Escherichia coli]
ATGGCATATCTGAAATTGATGAAGCTGCTCTATCTGTCTAACCGCAAGTCGATTTTGAAGCATGGCAGGATGATCGGCGA
AGATAGCCTTTACTCTATGAAATTTGGTCCAGTCATGTCGAATACGCTGAACTTGATTCGTGGTAAGGCTGAAGGTATTG
GTGACTACTGGTACAACCTGATAGAGACGAACGGGCATGATGTGTTGTTGCGTTCAGATCCGAGGGAAATGGATGCAGAC
GAGGTCTTTGATGAGTTGAGCCGTGCAGATATCCGGATTTTAGATGAAATCTATTCTCTGTATGGGCATATGAACCGATT
TGATCTCGCAAACATGACGCATTTAGAAAGCGTTTGTCCAGAGTGGCACAATCCTGGCAATTCTCGTAAGCCTATAGACC
TGAAAGAAATGCTGATCAGTGAGGGTAAAAGCGAGGATGAGGCTAATCGTATAATTGGCAAAATGGAAGAATCTCAGAAA
CTTAAGGAATTTTCTTTGCAATTATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W4B4V0

Antitoxin

Source ID Structure
AlphaFold DB A0A2W6NI96

References