Detailed information of TA system TA159975

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 944711..945337
• Replicon: plasmid unnamed4
• Accession: NZ_CP054613
• Organism: Paenibacillus cellulosilyticus strain KACC 14175

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7X3GLC0
• Locus tag: HUB94_RS23325
• Protein ID: WP_040711809.1
• Coordinates: 944711..945061 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUB94_RS23330
• Protein ID: WP_174812942.1
• Coordinates: 945065..945337 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUB94_RS23310	941867..943300	+	1434	WP_110046397.1	ammonium transporter	-
HUB94_RS23315	943307..943654	+	348	WP_110046396.1	P-II family nitrogen regulator	-
HUB94_RS23320	943779..944465	-	687	WP_110046395.1	type 1 glutamine amidotransferase	-
HUB94_RS23325	944711..945061	-	351	WP_040711809.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUB94_RS23330	945065..945337	-	273	WP_174812942.1	ribbon-helix-helix protein, CopG family	Antitoxin
HUB94_RS23335	945585..946763	-	1179	WP_110046393.1	alanine racemase	-
HUB94_RS23340	947030..948241	-	1212	WP_110046392.1	outer membrane lipoprotein carrier protein LolA	-
HUB94_RS23345	949160..949435	+	276	WP_110046391.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	clpP / groEL / dhbE / bpsC / clpP / tufA / clpC / bpsC	1..2697188	2697188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12894.85 Da
• Isoelectric Point: 4.8837

>T159975 WP_040711809.1 NZ_CP054613:c945061-944711 [Paenibacillus cellulosilyticus]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIEAESHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDDEMMRKVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T159975 NZ_CP070229:c2395927-2395754 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10448.11 Da
• Isoelectric Point: 7.0153

>AT159975 WP_174812942.1 NZ_CP054613:c945337-945065 [Paenibacillus cellulosilyticus]
MQNTKRIMISLPDQLLEEVDGIVAKENSNRSEFIRQAMKLYLMERKKRQIRESMQRGYMEMAKINLNMASEAFHAEEDAD
LTLGRLVSGV

Nucleotide

• Length: 273 bp

>AT159975 NZ_CP070229:c2396251-2395955 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7X3GLC0