Detailed information of TA system TA159971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 112500..113997
• Replicon: plasmid unnamed3
• Accession: NZ_CP054602
• Organism: Sulfitobacter pseudonitzschiae strain H46

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: A0A073J7K3
• Locus tag: HT745_RS20255
• Protein ID: WP_037931910.1
• Coordinates: 112500..113390 (-)
• Length: 297 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: A0A073ITP2
• Locus tag: HT745_RS20260
• Protein ID: WP_037931913.1
• Coordinates: 113383..113997 (-)
• Length: 205 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT745_RS20235	108298..109860	-	1563	WP_037931902.1	L-aspartate oxidase	-
HT745_RS20240	109865..110917	-	1053	WP_037931904.1	quinolinate synthase NadA	-
HT745_RS20245	111187..111471	+	285	WP_037931907.1	Dabb family protein	-
HT745_RS20250	111808..112005	+	198	WP_073194470.1	hypothetical protein	-
HT745_RS20255	112500..113390	-	891	WP_037931910.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HT745_RS20260	113383..113997	-	615	WP_037931913.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
HT745_RS20265	114389..114727	-	339	WP_037931919.1	hypothetical protein	-
HT745_RS20270	115219..116103	+	885	WP_073194473.1	DUF1738 domain-containing protein	-
HT745_RS20275	116377..116628	-	252	Protein_123	ABC transporter substrate-binding protein	-
HT745_RS20280	116639..117595	-	957	WP_037929714.1	HesA/MoeB/ThiF family protein	-
HT745_RS20285	117592..118188	-	597	WP_037929711.1	thiamine phosphate synthase	-
HT745_RS20290	118185..118955	-	771	WP_037929708.1	thiazole synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..120734	120734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-265)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (16-56)

Sequences

Toxin

Protein

• Length: 297 a.a.
• Molecular weight: 32552.00 Da
• Isoelectric Point: 4.9714

>T159971 WP_037931910.1 NZ_CP054602:c113390-112500 [Sulfitobacter pseudonitzschiae]
MVREIKNMGASVRARLLKISKDSEQNYQLVLTRYANERLLYRLAKSAHAERFVLKGAVLLMAWFDEPFRGTRDVDLLGHG
DPDPSAVLGVFKDIFAGDAPDGVQFDADGAQIGRIREDTEYGGLRIKTTANVGGARVSISVDVGFGDAMEPEPQILDLPG
LLDMPTAPLRGYARETVVAEKFQAMVALGLTNTRIKDYYDVWLLSRSFEFDEAQLGRAIAATFERRDTAVPTEAPDALTA
AFSKDEAKQRQWAAFIRDVSFDPGPLPGVVETLAGFLMPAAVTAMRHKPQSKDDQS

Nucleotide

• Length: 891 bp

>T159971 NZ_CP070229:1054085-1054363 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACCAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTGGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 205 a.a.
• Molecular weight: 22243.56 Da
• Isoelectric Point: 10.1653

>AT159971 WP_037931913.1 NZ_CP054602:c113997-113383 [Sulfitobacter pseudonitzschiae]
MPGRKTQREQAKEMLWDRGIVRLSEFKSAGITAATIGRMRDDGDVIRLARGLYQLPDAPLDANHSLAEAAKRVPKGVVCL
TSALAFHELTDQLPRSVWMAIGKNDWSPQDEPALRIVRFTDTLLAQDVKTTSIEGVPVKVFAVAKTIADCFRHRRSVGQS
VALEGLQEALRQRKATPAEIARSAASGGVSTIVRPYLEALTANG

Nucleotide

• Length: 615 bp

>AT159971 NZ_CP070229:1053892-1054083 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAGAATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A073J7K3

Antitoxin

• AlphaFold DB: A0A073ITP2