Detailed information of TA system TA159966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1929402..1930041
• Replicon: chromosome
• Accession: NZ_CP054596
• Organism: Bifidobacterium longum subsp. infantis strain PI_006

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: HUE65_RS08375
• Protein ID: WP_014484977.1
• Coordinates: 1929402..1929758 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE65_RS08380
• Protein ID: WP_003829235.1
• Coordinates: 1929745..1930041 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE65_RS08345 (HUE65_08360)	1924865..1925857	+	993	WP_080714690.1	ABC transporter ATP-binding protein	-
HUE65_RS08350 (HUE65_08365)	1926056..1926682	+	627	WP_007052130.1	30S ribosomal protein S4	-
HUE65_RS08355 (HUE65_08370)	1926861..1927643	-	783	WP_032743690.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HUE65_RS08360 (HUE65_08375)	1927781..1928170	-	390	WP_230581178.1	cytidine deaminase	-
HUE65_RS08365 (HUE65_08380)	1928187..1928420	+	234	WP_235625961.1	hypothetical protein	-
HUE65_RS08370 (HUE65_08385)	1928525..1929127	+	603	WP_032743691.1	transglutaminase family protein	-
HUE65_RS08375 (HUE65_08390)	1929402..1929758	-	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE65_RS08380 (HUE65_08395)	1929745..1930041	-	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE65_RS08385 (HUE65_08400)	1930111..1930377	-	267	WP_230581180.1	hypothetical protein	-
HUE65_RS08390 (HUE65_08405)	1930452..1931126	+	675	WP_032743692.1	histidine phosphatase family protein	-
HUE65_RS08395 (HUE65_08410)	1931209..1931607	+	399	WP_032743693.1	DUF948 domain-containing protein	-
HUE65_RS08400 (HUE65_08415)	1931607..1931843	+	237	WP_007053386.1	hypothetical protein	-
HUE65_RS08405 (HUE65_08420)	1931972..1934650	+	2679	WP_032743694.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T159966 WP_014484977.1 NZ_CP054596:c1929758-1929402 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T159966 NZ_CP070229:206695-206797 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT159966 WP_003829235.1 NZ_CP054596:c1930041-1929745 [Bifidobacterium longum subsp. infantis]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT159966 NZ_CP070229:c206835-206567 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• AlphaFold DB: A0A0L7D489