Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 519268..519904 Replicon chromosome
Accession NZ_CP054584
Organism Bacillus halotolerans strain KKD1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HT135_RS02605 Protein ID WP_003156187.1
Coordinates 519554..519904 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag HT135_RS02600 Protein ID WP_003225183.1
Coordinates 519268..519549 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT135_RS02580 515623..516222 - 600 WP_024120294.1 rhomboid family intramembrane serine protease -
HT135_RS02585 516317..516682 + 366 WP_106020948.1 holo-ACP synthase -
HT135_RS02590 516848..517864 + 1017 WP_106021112.1 outer membrane lipoprotein carrier protein LolA -
HT135_RS02595 517981..519150 + 1170 WP_024120297.1 alanine racemase -
HT135_RS02600 519268..519549 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HT135_RS02605 519554..519904 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HT135_RS02610 520020..520844 + 825 WP_081638235.1 RsbT co-antagonist protein RsbRA -
HT135_RS02615 520849..521214 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
HT135_RS02620 521217..521618 + 402 WP_106020946.1 serine/threonine-protein kinase RsbT -
HT135_RS02625 521630..522637 + 1008 WP_024120300.1 phosphoserine phosphatase RsbU -
HT135_RS02630 522698..523027 + 330 WP_024120301.1 anti-sigma factor antagonist RsbV -
HT135_RS02635 523024..523506 + 483 WP_024120302.1 anti-sigma B factor RsbW -
HT135_RS02640 523472..524260 + 789 WP_024120303.1 RNA polymerase sigma factor SigB -
HT135_RS02645 524260..524859 + 600 WP_024120304.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T159945 WP_003156187.1 NZ_CP054584:519554-519904 [Bacillus halotolerans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T159945 NZ_CP070227:470542-470715 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT159945 WP_003225183.1 NZ_CP054584:519268-519549 [Bacillus halotolerans]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT159945 NZ_CP070227:470218-470514 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References