Detailed information of TA system TA159943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5837877..5838463
• Replicon: chromosome
• Accession: NZ_CP054581
• Organism: Pseudomonas aeruginosa strain YTSEY8

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HUF04_RS27760
• Protein ID: WP_003120987.1
• Coordinates: 5838164..5838463 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HUF04_RS27755
• Protein ID: WP_003448662.1
• Coordinates: 5837877..5838167 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF04_RS27735	5833028..5833237	+	210	WP_003105733.1	cold-shock protein	-
HUF04_RS27740	5833459..5835348	+	1890	WP_016851610.1	hypothetical protein	-
HUF04_RS27745	5835345..5837321	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HUF04_RS27750	5837462..5837806	+	345	WP_016851612.1	hypothetical protein	-
HUF04_RS27755	5837877..5838167	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HUF04_RS27760	5838164..5838463	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HUF04_RS27765	5838665..5839789	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HUF04_RS27770	5839789..5841498	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
HUF04_RS27775	5841502..5842827	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HUF04_RS27780	5842817..5843350	+	534	WP_202096777.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pilC / xcpA/pilD	5793313..5914441	121128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T159943 WP_003120987.1 NZ_CP054581:c5838463-5838164 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T159943 NZ_CP070223:c489722-488859 [Hydrogenophaga sp. YM1]
ATGAGTGCGGCTACCCCAGCTGTCGCGGCATCCGTCCGTGCGCGTCTGCTCAATGTCGCCAAGGCGCAAGGCGTCGATTT
CAATCAGGTGCTGGTGCGCTTCGCCTTGGAGCGCATCCTCTACCGGCTGACCCAGTCGCAGCACGCGGACCGCTTTCTGC
TCAAAGGTGCGCTGCTGTTCACGCTCTGGTACGACATGCCACACCGGGCCACACGCGATGCCGACCTCTTGGGTTTCGGT
GCCAGTGATCTGGAGTCAGTGGCCCAAGTATTCCGGGACATCGCAGCTGTACCAGTCGACGACGGCATTGTGTTCGACCC
AGCCTCCGTCACGGTCGAGGAGATCCGCAAGGAAGCTGGCTACGGTGGCGTTCGCGTGATGATTGCAGGCGACCTGGCCA
GAGCACGCTGCAAGACCCAAATCGATGTCGGCTTCGGCGACGCCGTCACCCCTGCGCCTGTTGATTCGGTCTACCCAGTA
CTGCTGGAGGAAATGCCCGCCCCACGGCTGCGGGCCTACCCAACGTACACCGTCATCGCGGAAAAGCTCCACGCCATCGC
GTTGCTGGGCATGACCAACAGCCGCTTGAAGGACTACTTCGATCTGTCTGTGCTGTTGGAACGAGAAACTCTGGACACTG
ATCTGCTGGCCCAGGCGATCAAGGCCACGTTCGAAAGACGGGGCATGGCGCTTCCTGATGCGCTGCCAGTCGGCCTGACG
GACGAGTTTGCGCACGACGCTTCGCGCCAGGCGCTGTGGCTGGCATTTCTCAAGAAGAACGAACTTCCGCCAGAACCCTT
GGCCGCCATCGTTGGCCGCTTGAGCGTGGCATTGGCACCCGCGTTGAACACCGTCGCTCGTTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT159943 WP_003448662.1 NZ_CP054581:c5838167-5837877 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT159943 NZ_CP070223:c490324-489719 [Hydrogenophaga sp. YM1]
ATGCTCTCCAGTACCCACAATCAACGCGTCCTCGATCTGGCCAGCCAGAAGGGGCTACTGCGCGCCAGCGATCTGGACGC
CATCAGTGCGCCTCGGGTCGTTCTGACGCGCCTGACCGCTGCGGGCCTACTAGAGAAGGTGGGGCGTGGCCTGTACCGTC
TGCCGGATGCACAGGTTTCGGAATTCGAGAGCCTTGGCACCATCGCCACCAAGGTACCTCAAGCGGTGTTCTGCCTGCTT
ACGGCGCTGCAGTTTCATGAGCTGACCACCCAGCTGCCGCGTCAGATCTGGATCGCCATGCCTCGGGGCAGCCACCCACC
CCGGGTCGACTACCCGCCCGTCAAGATGGTGCAGTTCACGGGCGATGCCTACTCGGCAGGGATTGAGGAAGTCGAACGCG
ACGGTGTCAGGCTCCGGGTGTACGGCGTGGCCAAGACCGTGGCGGACTGCTTCAAGCACCGCAACAAGATCGGGCTGGAT
GTGGCGCTGGAGGCGCTGAAGGACGCTCGGGCGCGCAGCAAGGCCTCCGTCGACGACATCTGGCGATTCGCCAAGATATG
CCGCGTGGCCAATGTGATGCGCCCCTATCTGGAGAGCGTTGGATGA

Structures

Toxin

• AlphaFold DB: G8CP73