Detailed information of TA system TA159940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4028708..4029929
• Replicon: chromosome
• Accession: NZ_CP054581
• Organism: Pseudomonas aeruginosa strain YTSEY8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUF04_RS19090
• Protein ID: WP_003162864.1
• Coordinates: 4029351..4029929 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUF04_RS19085
• Protein ID: WP_003088112.1
• Coordinates: 4028708..4029262 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF04_RS19060	4024276..4024827	+	552	WP_003102727.1	XRE family transcriptional regulator	-
HUF04_RS19065	4024824..4025222	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HUF04_RS19070	4025235..4025558	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
HUF04_RS19075	4025889..4026350	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HUF04_RS19080	4026355..4028550	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HUF04_RS19085	4028708..4029262	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
HUF04_RS19090	4029351..4029929	+	579	WP_003162864.1	HD domain-containing protein	Toxin
HUF04_RS19095	4030037..4031224	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HUF04_RS19100	4031214..4033385	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
HUF04_RS19105	4033375..4034652	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21911.78 Da
• Isoelectric Point: 5.3655

>T159940 WP_003162864.1 NZ_CP054581:4029351-4029929 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDILRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T159940 NZ_CP070173:86541-86846 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGGTCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT159940 WP_003088112.1 NZ_CP054581:4028708-4029262 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT159940 NZ_CP070173:86321-86539 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA