Detailed information of TA system TA159939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2554846..2555640
• Replicon: chromosome
• Accession: NZ_CP054581
• Organism: Pseudomonas aeruginosa strain YTSEY8

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A844NUK8
• Locus tag: HUF04_RS12270
• Protein ID: WP_021263385.1
• Coordinates: 2555461..2555640 (-)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HUF04_RS12265
• Protein ID: WP_021263386.1
• Coordinates: 2554846..2555421 (-)
• Length: 192 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF04_RS12230	2550104..2550676	-	573	WP_023088260.1	S24 family peptidase	-
HUF04_RS12235	2551054..2551272	+	219	WP_016561979.1	helix-turn-helix domain-containing protein	-
HUF04_RS12240	2551304..2551876	+	573	WP_019682191.1	hypothetical protein	-
HUF04_RS12245	2551879..2552349	+	471	Protein_2420	hypothetical protein	-
HUF04_RS12250	2552813..2553421	+	609	WP_021263389.1	hypothetical protein	-
HUF04_RS12255	2553414..2553857	+	444	WP_021263388.1	RusA family crossover junction endodeoxyribonuclease	-
HUF04_RS12260	2553886..2554755	+	870	WP_021263387.1	hypothetical protein	-
HUF04_RS12265	2554846..2555421	-	576	WP_021263386.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HUF04_RS12270	2555461..2555640	-	180	WP_021263385.1	type II toxin-antitoxin system HicA family toxin	Toxin
HUF04_RS12275	2555729..2555944	+	216	WP_019486654.1	hypothetical protein	-
HUF04_RS12280	2556087..2556476	+	390	WP_004349441.1	hypothetical protein	-
HUF04_RS12285	2556469..2556753	+	285	WP_016852757.1	phage holin family protein	-
HUF04_RS12290	2556762..2557358	+	597	WP_021263384.1	terminase small subunit	-
HUF04_RS12295	2557345..2558640	+	1296	WP_021263383.1	terminase family protein	-
HUF04_RS12300	2558643..2559998	+	1356	WP_021263382.1	DUF4055 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2521467..2585606	64139

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6591.62 Da
• Isoelectric Point: 11.1475

>T159939 WP_021263385.1 NZ_CP054581:c2555640-2555461 [Pseudomonas aeruginosa]
MKFSEFRRWLRAQGVSFEAGKGSHFKVTAPNGKTTTFADHGAKEMPEPTRKAIIKQLGL

Nucleotide

• Length: 180 bp

>T159939 NZ_CP070173:c85506-85090 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 192 a.a.
• Molecular weight: 21294.78 Da
• Isoelectric Point: 8.3556

>AT159939 WP_021263386.1 NZ_CP054581:c2555421-2554846 [Pseudomonas aeruginosa]
MYNFAIRFEQDDSAPGVAVFCRDLPELNSYGDTVEHALSEAVDAIETTLSLYVDQRRSIPVASAPEPEEHVVHLPAVTVA
KIALWNEMMRRGMRKADLCRVLGIAQVQGDRLVDFLHTSKMEQVESALAALEMRLVICAEPVKQPKQCKGAGCIRDRRLV
KARRLLRARLLELTESSGAFTRAPKIKKPGI

Nucleotide

• Length: 576 bp

>AT159939 NZ_CP070173:c85733-85503 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A844NUK8