Detailed information of TA system TA159934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 5312227..5312894
• Replicon: chromosome
• Accession: NZ_CP054580
• Organism: Halomonas titanicae strain GPM3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A6N0Z796
• Locus tag: FX987_RS24775
• Protein ID: WP_022519783.1
• Coordinates: 5312478..5312894 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A558JBE0
• Locus tag: FX987_RS24770
• Protein ID: WP_022519784.1
• Coordinates: 5312227..5312481 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX987_RS24730	5307417..5307941	+	525	WP_022519893.1	hypothetical protein	-
FX987_RS24735	5307976..5308158	+	183	WP_022519892.1	hypothetical protein	-
FX987_RS24740	5308199..5308573	-	375	WP_022519891.1	GFA family protein	-
FX987_RS24745	5308864..5309237	+	374	Protein_4865	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
FX987_RS24750	5309227..5309720	+	494	Protein_4866	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
FX987_RS24755	5309816..5310766	+	951	WP_022519887.1	DUF808 domain-containing protein	-
FX987_RS24760	5311119..5311427	-	309	WP_039858818.1	hypothetical protein	-
FX987_RS24765	5311811..5312116	-	306	WP_022519785.1	hypothetical protein	-
FX987_RS24770	5312227..5312481	+	255	WP_022519784.1	Arc family DNA-binding protein	Antitoxin
FX987_RS24775	5312478..5312894	+	417	WP_022519783.1	type II toxin-antitoxin system VapC family toxin	Toxin
FX987_RS24780	5313434..5313778	+	345	Protein_4872	TRAP transporter substrate-binding protein DctP	-
FX987_RS24785	5314193..5315533	+	1341	WP_170834172.1	group II intron reverse transcriptase/maturase	-
FX987_RS24790	5315616..5316290	+	675	Protein_4874	DctP family TRAP transporter solute-binding subunit	-
FX987_RS24795	5316375..5316878	+	504	WP_022519731.1	TRAP transporter small permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14831.96 Da
• Isoelectric Point: 4.3331

>T159934 WP_022519783.1 NZ_CP054580:5312478-5312894 [Halomonas titanicae]
MIVLDTNVVSEAMKPEPDPAVRAWLNEQSAETLYLSSVTLAELLFGIAALPNGKRKDMLSETLGGLVELFRGRILSFDVD
AARKYAELAVTARTAGRGFPVPDGYIAAIAVSQGYQVASRDMAPFEAANVDVINPFEA

Nucleotide

• Length: 417 bp

>T159934 NZ_CP070172:c2114078-2113860 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9487.92 Da
• Isoelectric Point: 7.2067

>AT159934 WP_022519784.1 NZ_CP054580:5312227-5312481 [Halomonas titanicae]
MGMMTIRSIPDEVHNALKARAKQNHRSAEAEVRAILEEITRPESRLKMGDALADLGRKIGLTNEDVAVFEKVRDKTPAEP
MRFE

Nucleotide

• Length: 255 bp

>AT159934 NZ_CP070172:c2114479-2114105 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A6N0Z796

Antitoxin

• AlphaFold DB: A0A558JBE0