Detailed information of TA system TA159914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 828100..828629
• Replicon: chromosome
• Accession: NZ_CP054575
• Organism: Staphylococcus saprophyticus strain UTI-050

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q49Z22
• Locus tag: HSZ50_RS03985
• Protein ID: WP_011302703.1
• Coordinates: 828267..828629 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q49Z23
• Locus tag: HSZ50_RS03980
• Protein ID: WP_011302702.1
• Coordinates: 828100..828270 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HSZ50_RS03955	823831..824310	+	480	WP_011302698.1	PH domain-containing protein	-
HSZ50_RS03960	824303..825841	+	1539	WP_048787404.1	PH domain-containing protein	-
HSZ50_RS03965	825834..826334	+	501	WP_011302700.1	PH domain-containing protein	-
HSZ50_RS03970	826398..826748	+	351	WP_002482750.1	holo-ACP synthase	-
HSZ50_RS03975	826864..828012	+	1149	WP_069794559.1	alanine racemase	-
HSZ50_RS03980	828100..828270	+	171	WP_011302702.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
HSZ50_RS03985	828267..828629	+	363	WP_011302703.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HSZ50_RS03990	828966..829967	+	1002	WP_011302704.1	PP2C family protein-serine/threonine phosphatase	-
HSZ50_RS03995	830046..830372	+	327	WP_011302705.1	anti-sigma factor antagonist	-
HSZ50_RS04000	830374..830856	+	483	WP_002482755.1	anti-sigma B factor RsbW	-
HSZ50_RS04005	830831..831601	+	771	WP_002482756.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13482.70 Da
• Isoelectric Point: 10.3173

>T159914 WP_011302703.1 NZ_CP054575:828267-828629 [Staphylococcus saprophyticus]
MMRRGDVYLADLSPVQGSEQGGIRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKSKYKLDKDSVILLEQIR
TLDKNRLKEKLTYLSDKKMKEVNNAIDISLGLHTIRSHKA

Nucleotide

• Length: 363 bp

>T159914 NZ_CP070170:2441765-2442043 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6332.00 Da
• Isoelectric Point: 4.0121

>AT159914 WP_011302702.1 NZ_CP054575:828100-828270 [Staphylococcus saprophyticus]
MASFNQSRIHNIEQLLKEGYSQMADLNLSLAAEAFPFECEACDCNEVYISSKNNNE

Nucleotide

• Length: 171 bp

>AT159914 NZ_CP070170:2442043-2442327 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: Q49Z22

Antitoxin

• AlphaFold DB: Q49Z23