Detailed information of TA system TA159908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 474687..475327
• Replicon: chromosome
• Accession: NZ_CP054572
• Organism: Pseudomonas sp. FDAARGOS_761

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOC67_RS02235
• Protein ID: WP_003105740.1
• Coordinates: 474917..475327 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6N0KGL5
• Locus tag: FOC67_RS02230
• Protein ID: WP_023132185.1
• Coordinates: 474687..474917 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC67_RS02220	471689..472441	-	753	WP_009315847.1	hypothetical protein	-
FOC67_RS02225	472449..474368	-	1920	WP_031627730.1	type I DNA topoisomerase	-
FOC67_RS02230	474687..474917	+	231	WP_023132185.1	antitoxin	Antitoxin
FOC67_RS02235	474917..475327	+	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
FOC67_RS02240	475349..475609	+	261	WP_003105742.1	hypothetical protein	-
FOC67_RS02245	475809..476027	+	219	WP_003105747.1	hypothetical protein	-
FOC67_RS02250	476093..476260	-	168	WP_128729268.1	CrpP family protein	-
FOC67_RS02255	476424..476912	-	489	WP_003105748.1	single-stranded DNA-binding protein	-
FOC67_RS02260	476942..477772	-	831	WP_009315858.1	phage regulatory protein	-
FOC67_RS02265	477820..478365	-	546	WP_009315860.1	DUF3158 family protein	-
FOC67_RS02270	478371..479099	-	729	WP_009315862.1	TIGR03761 family integrating conjugative element protein	-
FOC67_RS02275	479256..479453	-	198	WP_009315864.1	hypothetical protein	-
FOC67_RS02280	479365..479690	-	326	Protein_441	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	378848..494580	115732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T159908 WP_003105740.1 NZ_CP054572:474917-475327 [Pseudomonas sp. FDAARGOS_761]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T159908 NZ_CP070170:1099933-1100244 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8468.35 Da
• Isoelectric Point: 4.2821

>AT159908 WP_023132185.1 NZ_CP054572:474687-474917 [Pseudomonas sp. FDAARGOS_761]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTSDFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT159908 NZ_CP070170:1100241-1100660 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N0KGL5