Detailed information of TA system TA159899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2171301..2171971
• Replicon: chromosome
• Accession: NZ_CP054569
• Organism: Achromobacter denitrificans strain FDAARGOS_787

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FOC81_RS10345
• Protein ID: WP_174716226.1
• Coordinates: 2171546..2171971 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOC81_RS10340
• Protein ID: WP_089031316.1
• Coordinates: 2171301..2171549 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC81_RS10315	2166395..2166622	+	228	WP_089031311.1	AlpA family phage regulatory protein	-
FOC81_RS10320	2166624..2167475	+	852	WP_174716222.1	helicase RepA family protein	-
FOC81_RS10325	2167462..2168226	+	765	WP_174716223.1	ABC transporter ATPase	-
FOC81_RS10330	2169033..2169404	+	372	WP_174716224.1	conjugal transfer transcriptional regulator TraJ	-
FOC81_RS10335	2169439..2171235	+	1797	WP_174716225.1	relaxase/mobilization nuclease domain-containing protein	-
FOC81_RS10340	2171301..2171549	+	249	WP_089031316.1	Arc family DNA-binding protein	Antitoxin
FOC81_RS10345	2171546..2171971	+	426	WP_174716226.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC81_RS10350	2172162..2172938	+	777	WP_086068259.1	P-type conjugative transfer protein TrbJ	-
FOC81_RS10355	2172941..2173114	+	174	WP_172400410.1	hypothetical protein	-
FOC81_RS10360	2173117..2174556	+	1440	WP_174716227.1	P-type conjugative transfer protein TrbL	-
FOC81_RS10365	2174561..2174800	+	240	WP_174716228.1	hypothetical protein	-
FOC81_RS10370	2174865..2175095	+	231	WP_174716229.1	type I toxin-antitoxin system ptaRNA1 family toxin	-
FOC81_RS10375	2175193..2175447	-	255	WP_174716230.1	hypothetical protein	-
FOC81_RS10380	2175444..2175608	-	165	WP_174716231.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
FOC81_RS10385	2175788..2176360	+	573	WP_174716232.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

• Arc-like DNA binding domain (9-46)

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15129.42 Da
• Isoelectric Point: 4.4066

>T159899 WP_174716226.1 NZ_CP054569:2171546-2171971 [Achromobacter denitrificans]
MILLDTNVISEQFRAVPDETVIGWLNRQPLETLYLASMTVAELRAGVALMPAGKRRTVLSDSIEHQVLPVFVGRVLSFDM
ACTRAYADVLAAVRKAGSGIEAADAVIAAIALESGFSVATRDVSPFLAAGVNVINPWEDQE

Nucleotide

• Length: 426 bp

>T159899 NZ_CP070169:c3924258-3923875 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9082.50 Da
• Isoelectric Point: 9.3441

>AT159899 WP_089031316.1 NZ_CP054569:2171301-2171549 [Achromobacter denitrificans]
MRAVTIRNVPEEVHRAIRVRAAQNGRTLQAEMCEILATAVKPEGRVKLGDLLAGIGRKVKLTDEEMAVFERDHSPARAAS
FE

Nucleotide

• Length: 249 bp

>AT159899 NZ_CP070169:c3924500-3924258 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA