Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2671382..2672024 Replicon chromosome
Accession NZ_CP054568
Organism Bacillus thuringiensis strain FDAARGOS_791

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC85_RS14180 Protein ID WP_000635963.1
Coordinates 2671674..2672024 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC85_RS14175 Protein ID WP_000004570.1
Coordinates 2671382..2671669 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC85_RS14150 2666698..2667660 + 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -
FOC85_RS14155 2667653..2668225 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC85_RS14160 2668318..2668677 + 360 WP_000635040.1 holo-ACP synthase -
FOC85_RS14165 2668834..2669784 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC85_RS14170 2669903..2671072 + 1170 WP_000390600.1 alanine racemase -
FOC85_RS14175 2671382..2671669 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC85_RS14180 2671674..2672024 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC85_RS14185 2672092..2674260 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC85_RS14190 2674318..2674434 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC85_RS14195 2674630..2675088 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T159897 WP_000635963.1 NZ_CP054568:2671674-2672024 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T159897 NZ_CP070169:c3446030-3445746 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT159897 WP_000004570.1 NZ_CP054568:2671382-2671669 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT159897 NZ_CP070169:c3446262-3446020 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References