Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3391969..3392600 Replicon chromosome
Accession NZ_CP054556
Organism Escherichia coli strain LWY24

Toxin (Protein)


Gene name hicA Uniprot ID L4J012
Locus tag HT750_RS16315 Protein ID WP_001260301.1
Coordinates 3391969..3392244 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HT750_RS16320 Protein ID WP_000593555.1
Coordinates 3392241..3392600 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT750_RS16300 3386973..3388040 + 1068 WP_000361497.1 HlyD family efflux transporter periplasmic adaptor subunit -
HT750_RS16305 3388037..3390772 + 2736 WP_061092822.1 ribosome-associated ATPase/putative transporter RbbA -
HT750_RS16310 3390772..3391896 + 1125 WP_001314210.1 ABC transporter permease -
HT750_RS16315 3391969..3392244 + 276 WP_001260301.1 type II toxin-antitoxin system HicA family toxin Toxin
HT750_RS16320 3392241..3392600 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HT750_RS16325 3392720..3393121 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HT750_RS16330 3393126..3393932 - 807 WP_061092823.1 nickel import ATP-binding protein NikE -
HT750_RS16335 3393929..3394693 - 765 WP_001136211.1 nickel import ATP-binding protein NikD -
HT750_RS16340 3394693..3395526 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HT750_RS16345 3395523..3396467 - 945 WP_000947080.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10185.87 Da        Isoelectric Point: 10.6128

>T159885 WP_001260301.1 NZ_CP054556:3391969-3392244 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T159885 NZ_CP070162:c2646377-2646126 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT159885 WP_000593555.1 NZ_CP054556:3392241-3392600 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT159885 NZ_CP070162:c2646129-2645875 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCACTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB L4J012

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References