Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2327377..2327953 Replicon chromosome
Accession NZ_CP054556
Organism Escherichia coli strain LWY24

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HT750_RS11440 Protein ID WP_001131965.1
Coordinates 2327666..2327953 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag HT750_RS11435 Protein ID WP_000063158.1
Coordinates 2327377..2327679 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT750_RS11415 2322485..2323705 + 1221 WP_000873756.1 amino acid deaminase -
HT750_RS11420 2323973..2326123 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HT750_RS11425 2326173..2326376 + 204 WP_000467859.1 YbdD/YjiX family protein -
HT750_RS11430 2326387..2327343 + 957 WP_001361361.1 GTPase -
HT750_RS11435 2327377..2327679 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
HT750_RS11440 2327666..2327953 - 288 WP_001131965.1 BrnT family toxin Toxin
HT750_RS11445 2328150..2329316 + 1167 WP_000800823.1 restriction endonuclease -
HT750_RS11450 2329361..2329534 + 174 Protein_2245 SAM-dependent DNA methyltransferase -
HT750_RS11455 2329528..2330196 + 669 Protein_2246 DUF3387 domain-containing protein -
HT750_RS11460 2330411..2330749 + 339 WP_000132633.1 endoribonuclease SymE -
HT750_RS11465 2330791..2330955 - 165 WP_000394263.1 DUF1127 domain-containing protein -
HT750_RS11470 2331132..2332544 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T159879 WP_001131965.1 NZ_CP054556:c2327953-2327666 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T159879 NZ_CP070162:c1694516-1694409 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT159879 WP_000063158.1 NZ_CP054556:c2327679-2327377 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT159879 NZ_CP070162:1694564-1694630 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References