159878

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1831600..1832437 Replicon chromosome
Accession NZ_CP054556
Organism Escherichia coli strain LWY24

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag HT750_RS09090 Protein ID WP_000227782.1
Coordinates 1831895..1832437 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag HT750_RS09085 Protein ID WP_001353405.1
Coordinates 1831600..1831911 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT750_RS09060 1826620..1827567 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
HT750_RS09065 1827589..1829580 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HT750_RS09070 1829570..1830184 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HT750_RS09075 1830184..1830513 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HT750_RS09080 1830525..1831415 + 891 WP_000971327.1 heme o synthase -
HT750_RS09085 1831600..1831911 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
HT750_RS09090 1831895..1832437 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
HT750_RS09095 1832493..1833337 - 845 Protein_1785 sel1 repeat family protein -
HT750_RS09100 1833744..1835108 + 1365 WP_001000978.1 MFS transporter -
HT750_RS09105 1835236..1835727 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HT750_RS09110 1835895..1836806 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
HT750_RS09115 1836769..1837359 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T159878 WP_000227782.1 NZ_CP054556:1831895-1832437 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T159878 NZ_CP070162:976622-976924 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT159878 WP_001353405.1 NZ_CP054556:1831600-1831911 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT159878 NZ_CP070162:976960-977301 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References