Detailed information of TA system TA159875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1369754..1370459
• Replicon: chromosome
• Accession: NZ_CP054556
• Organism: Escherichia coli strain LWY24

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: HT750_RS06780
• Protein ID: WP_000539524.1
• Coordinates: 1369754..1370140 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HT750_RS06785
• Protein ID: WP_001280945.1
• Coordinates: 1370130..1370459 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT750_RS06760	1365758..1366384	+	627	WP_001295292.1	glutathione S-transferase GstB	-
HT750_RS06765	1366381..1367496	-	1116	WP_174617247.1	aldose sugar dehydrogenase YliI	-
HT750_RS06770	1367607..1367990	-	384	WP_000497137.1	biofilm formation regulator BssR	-
HT750_RS06775	1368203..1369528	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HT750_RS06780	1369754..1370140	+	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HT750_RS06785	1370130..1370459	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HT750_RS06790	1370529..1371846	-	1318	Protein_1340	diguanylate cyclase	-
HT750_RS06795	1371854..1374202	-	2349	WP_000950331.1	EAL domain-containing protein	-
HT750_RS06800	1374379..1375290	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T159875 WP_000539524.1 NZ_CP054556:1369754-1370140 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T159875 NZ_CP070162:516200-516514 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT159875 WP_001280945.1 NZ_CP054556:1370130-1370459 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT159875 NZ_CP070162:515964-516197 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7