Detailed information of TA system TA159864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 204607..205391
• Replicon: chromosome
• Accession: NZ_CP054556
• Organism: Escherichia coli strain LWY24

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: HT750_RS01040
• Protein ID: WP_000613626.1
• Coordinates: 204607..205101 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: HT750_RS01045
• Protein ID: WP_001110447.1
• Coordinates: 205098..205391 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT750_RS01020	199800..200897	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
HT750_RS01025	200897..201838	+	942	WP_001305885.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
HT750_RS01030	201904..203547	+	1644	WP_061092652.1	flagellar hook-associated protein FlgK	-
HT750_RS01035	203559..204512	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
HT750_RS01040	204607..205101	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
HT750_RS01045	205098..205391	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
HT750_RS01050	205524..208709	-	3186	WP_061092653.1	ribonuclease E	-
HT750_RS01055	209282..210241	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T159864 WP_000613626.1 NZ_CP054556:c205101-204607 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T159864 NZ_CP070158:1266116-1266400 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT159864 WP_001110447.1 NZ_CP054556:c205391-205098 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT159864 NZ_CP070158:1265884-1266126 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6