Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 171346..171895 Replicon chromosome
Accession NZ_CP054528
Organism Bifidobacterium longum subsp. infantis strain NLS

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag HUE59_RS00830 Protein ID WP_003829109.1
Coordinates 171572..171895 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M4LW25
Locus tag HUE59_RS00825 Protein ID WP_032743660.1
Coordinates 171346..171585 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE59_RS00805 (HUE59_00805) 167190..167852 + 663 WP_032743761.1 TetR/AcrR family transcriptional regulator -
HUE59_RS00810 (HUE59_00810) 168009..169280 + 1272 WP_032743663.1 MFS transporter -
HUE59_RS00815 169464..169574 - 111 WP_230581173.1 XRE family transcriptional regulator -
HUE59_RS00820 (HUE59_00820) 169910..171166 - 1257 WP_032743661.1 HipA domain-containing protein -
HUE59_RS00825 (HUE59_00825) 171346..171585 + 240 WP_032743660.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HUE59_RS00830 (HUE59_00830) 171572..171895 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HUE59_RS00835 (HUE59_00835) 172112..172726 - 615 WP_080714683.1 cupin domain-containing protein -
HUE59_RS00840 (HUE59_00840) 172874..173233 - 360 WP_080714682.1 class I SAM-dependent methyltransferase -
HUE59_RS00845 (HUE59_00845) 173471..173836 + 366 WP_032743659.1 YccF domain-containing protein -
HUE59_RS00850 (HUE59_00850) 174378..175643 + 1266 WP_050496214.1 Fic family protein -
HUE59_RS00855 (HUE59_00855) 176113..176402 + 290 Protein_174 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-54)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T159859 WP_003829109.1 NZ_CP054528:171572-171895 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T159859 NZ_CP070157:5030355-5030729 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTTAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9121.16 Da        Isoelectric Point: 4.5881

>AT159859 WP_032743660.1 NZ_CP054528:171346-171585 [Bifidobacterium longum subsp. infantis]
MQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDETR

Download         Length: 240 bp

>AT159859 NZ_CP070157:5029934-5030308 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGATATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCAGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4LW25

References