Detailed information of TA system TA159855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-RelB
• Location: 1601199..1601754
• Replicon: chromosome
• Accession: NZ_CP054527
• Organism: Bifidobacterium longum subsp. infantis strain BB-02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: S2ZXD7
• Locus tag: HUE60_RS07455
• Protein ID: WP_003829109.1
• Coordinates: 1601431..1601754 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE60_RS07450
• Protein ID: WP_212105461.1
• Coordinates: 1601199..1601444 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE60_RS07430 (HUE60_07395)	1597026..1597688	+	663	WP_019728044.1	TetR/AcrR family transcriptional regulator	-
HUE60_RS07435 (HUE60_07400)	1597845..1599116	+	1272	WP_025341702.1	MFS transporter	-
HUE60_RS07440 (HUE60_07405)	1599300..1599449	-	150	WP_234933099.1	XRE family transcriptional regulator	-
HUE60_RS07445 (HUE60_07410)	1599769..1601025	-	1257	WP_212105458.1	HipA domain-containing protein	-
HUE60_RS07450 (HUE60_07415)	1601199..1601444	+	246	WP_212105461.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE60_RS07455 (HUE60_07420)	1601431..1601754	+	324	WP_003829109.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE60_RS07460 (HUE60_07425)	1601971..1602585	-	615	WP_195338371.1	cupin domain-containing protein	-
HUE60_RS07465 (HUE60_07430)	1602680..1603549	-	870	WP_212105464.1	class I SAM-dependent methyltransferase	-
HUE60_RS07470 (HUE60_07435)	1603787..1604152	+	366	WP_032743659.1	YccF domain-containing protein	-
HUE60_RS07475 (HUE60_07440)	1604695..1605960	+	1266	WP_212105467.1	Fic family protein	-
HUE60_RS07480 (HUE60_07445)	1606430..1606728	+	299	Protein_1464	IS256 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-103)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-56)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11859.67 Da
• Isoelectric Point: 5.0771

>T159855 WP_003829109.1 NZ_CP054527:1601431-1601754 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Nucleotide

• Length: 324 bp

>T159855 NZ_CP070157:4299516-4300436 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9351.49 Da
• Isoelectric Point: 4.5881

>AT159855 WP_212105461.1 NZ_CP054527:1601199-1601444 [Bifidobacterium longum subsp. infantis]
MSMQIATRVDDEQAALFKETTRQLGITPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Nucleotide

• Length: 246 bp

>AT159855 NZ_CP070157:4298747-4299529 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A087B6Q7