Detailed information of TA system TA159854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1542909..1543548
• Replicon: chromosome
• Accession: NZ_CP054527
• Organism: Bifidobacterium longum subsp. infantis strain BB-02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HUE60_RS07170
• Protein ID: WP_212105396.1
• Coordinates: 1543192..1543548 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE60_RS07165
• Protein ID: WP_003829235.1
• Coordinates: 1542909..1543205 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE60_RS07140 (HUE60_07105)	1538300..1540978	-	2679	WP_212105390.1	alanine--tRNA ligase	-
HUE60_RS07145 (HUE60_07110)	1541107..1541343	-	237	WP_029679572.1	hypothetical protein	-
HUE60_RS07150 (HUE60_07115)	1541343..1541741	-	399	WP_012577919.1	DUF948 domain-containing protein	-
HUE60_RS07155 (HUE60_07120)	1541824..1542498	-	675	WP_212105393.1	histidine phosphatase family protein	-
HUE60_RS07160 (HUE60_07125)	1542574..1542762	+	189	WP_234933089.1	hypothetical protein	-
HUE60_RS07165 (HUE60_07130)	1542909..1543205	+	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE60_RS07170 (HUE60_07135)	1543192..1543548	+	357	WP_212105396.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE60_RS07175	1544006..1544341	-	336	WP_234933091.1	transglutaminase family protein	-
HUE60_RS07180 (HUE60_07145)	1544446..1544679	-	234	WP_248315138.1	hypothetical protein	-
HUE60_RS07185 (HUE60_07150)	1544696..1545085	+	390	WP_234933093.1	cytidine deaminase	-
HUE60_RS07190 (HUE60_07155)	1545223..1546005	+	783	WP_212105399.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HUE60_RS07195 (HUE60_07160)	1546343..1546969	-	627	WP_007052130.1	30S ribosomal protein S4	-
HUE60_RS07200 (HUE60_07165)	1547168..1548160	-	993	WP_212105402.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13315.26 Da
• Isoelectric Point: 7.9984

>T159854 WP_212105396.1 NZ_CP054527:1543192-1543548 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNHPSVILQWDDGTRIALMTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T159854 NZ_CP070157:4284413-4284811 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT159854 WP_003829235.1 NZ_CP054527:1542909-1543205 [Bifidobacterium longum subsp. infantis]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT159854 NZ_CP070157:4284117-4284410 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA