Detailed information of TA system TA159846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 112253..112892
• Replicon: chromosome
• Accession: NZ_CP054526
• Organism: Bifidobacterium longum subsp. infantis strain PI_002

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: HUE61_RS00540
• Protein ID: WP_014484977.1
• Coordinates: 112536..112892 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE61_RS00535
• Protein ID: WP_003829235.1
• Coordinates: 112253..112549 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE61_RS00510 (HUE61_00505)	107644..110322	-	2679	WP_032743694.1	alanine--tRNA ligase	-
HUE61_RS00515 (HUE61_00510)	110451..110687	-	237	WP_007053386.1	hypothetical protein	-
HUE61_RS00520 (HUE61_00515)	110687..111085	-	399	WP_032743693.1	DUF948 domain-containing protein	-
HUE61_RS00525 (HUE61_00520)	111168..111842	-	675	WP_032743692.1	histidine phosphatase family protein	-
HUE61_RS00530 (HUE61_00525)	111917..112183	+	267	WP_230581180.1	hypothetical protein	-
HUE61_RS00535 (HUE61_00530)	112253..112549	+	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE61_RS00540 (HUE61_00535)	112536..112892	+	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE61_RS00545 (HUE61_00540)	113167..113769	-	603	WP_032743691.1	transglutaminase family protein	-
HUE61_RS00550 (HUE61_00545)	113874..114149	-	276	WP_230581179.1	SdpI family protein	-
HUE61_RS00555 (HUE61_00550)	114124..114513	+	390	WP_230581178.1	cytidine deaminase	-
HUE61_RS00560 (HUE61_00555)	114651..115433	+	783	WP_032743690.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HUE61_RS00565 (HUE61_00560)	115612..116238	-	627	WP_007052130.1	30S ribosomal protein S4	-
HUE61_RS00570 (HUE61_00565)	116437..117429	-	993	WP_080714690.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T159846 WP_014484977.1 NZ_CP054526:112536-112892 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T159846 NZ_CP070157:c2647977-2647627 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT159846 WP_003829235.1 NZ_CP054526:112253-112549 [Bifidobacterium longum subsp. infantis]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT159846 NZ_CP070157:c2648222-2647971 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0L7D489