Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1671661..1672210 Replicon chromosome
Accession NZ_CP054525
Organism Bifidobacterium longum subsp. infantis strain PI_003

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag HUE62_RS07315 Protein ID WP_003829109.1
Coordinates 1671661..1671984 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M4LW25
Locus tag HUE62_RS07320 Protein ID WP_032743660.1
Coordinates 1671971..1672210 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE62_RS07290 (HUE62_07250) 1667154..1667443 - 290 Protein_1409 IS256 family transposase -
HUE62_RS07295 (HUE62_07255) 1667913..1669178 - 1266 WP_050496214.1 Fic family protein -
HUE62_RS07300 (HUE62_07260) 1669720..1670085 - 366 WP_032743659.1 YccF domain-containing protein -
HUE62_RS07305 (HUE62_07265) 1670323..1670682 + 360 WP_080714682.1 class I SAM-dependent methyltransferase -
HUE62_RS07310 (HUE62_07270) 1670830..1671444 + 615 WP_080714683.1 cupin domain-containing protein -
HUE62_RS07315 (HUE62_07275) 1671661..1671984 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HUE62_RS07320 (HUE62_07280) 1671971..1672210 - 240 WP_032743660.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HUE62_RS07325 (HUE62_07285) 1672390..1673646 + 1257 WP_032743661.1 HipA domain-containing protein -
HUE62_RS07330 (HUE62_07290) 1673964..1674092 + 129 WP_032743662.1 XRE family transcriptional regulator -
HUE62_RS07335 (HUE62_07295) 1674276..1675547 - 1272 WP_032743663.1 MFS transporter -
HUE62_RS07340 (HUE62_07300) 1675704..1676366 - 663 WP_032743761.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-54)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T159842 WP_003829109.1 NZ_CP054525:c1671984-1671661 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T159842 NZ_CP070157:c2004312-2003977 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9121.16 Da        Isoelectric Point: 4.5881

>AT159842 WP_032743660.1 NZ_CP054525:c1672210-1671971 [Bifidobacterium longum subsp. infantis]
MQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDETR

Download         Length: 240 bp

>AT159842 NZ_CP070157:c2004560-2004312 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4LW25

References