Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1550930..1551479 Replicon chromosome
Accession NZ_CP054523
Organism Bifidobacterium longum subsp. infantis strain PI_005

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag HUE64_RS07070 Protein ID WP_003829109.1
Coordinates 1551156..1551479 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M4LW25
Locus tag HUE64_RS07065 Protein ID WP_032743660.1
Coordinates 1550930..1551169 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE64_RS07045 (HUE64_07055) 1546774..1547436 + 663 WP_032743761.1 TetR/AcrR family transcriptional regulator -
HUE64_RS07050 (HUE64_07060) 1547593..1548864 + 1272 WP_032743663.1 MFS transporter -
HUE64_RS07055 1549048..1549158 - 111 WP_230581173.1 XRE family transcriptional regulator -
HUE64_RS07060 (HUE64_07070) 1549494..1550750 - 1257 WP_032743661.1 HipA domain-containing protein -
HUE64_RS07065 (HUE64_07075) 1550930..1551169 + 240 WP_032743660.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HUE64_RS07070 (HUE64_07080) 1551156..1551479 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HUE64_RS07075 (HUE64_07085) 1551696..1552310 - 615 WP_080714683.1 cupin domain-containing protein -
HUE64_RS07080 (HUE64_07090) 1552458..1552817 - 360 WP_080714682.1 class I SAM-dependent methyltransferase -
HUE64_RS07085 (HUE64_07095) 1553055..1553420 + 366 WP_032743659.1 YccF domain-containing protein -
HUE64_RS07090 (HUE64_07100) 1553962..1555227 + 1266 WP_050496214.1 Fic family protein -
HUE64_RS07095 (HUE64_07105) 1555697..1555986 + 290 Protein_1386 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-54)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T159831 WP_003829109.1 NZ_CP054523:1551156-1551479 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T159831 NZ_CP070152:c4363685-4363299 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9121.16 Da        Isoelectric Point: 4.5881

>AT159831 WP_032743660.1 NZ_CP054523:1550930-1551169 [Bifidobacterium longum subsp. infantis]
MQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDETR

Download         Length: 240 bp

>AT159831 NZ_CP070152:c4363309-4362980 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4LW25

References