Detailed information of TA system TA159830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1491837..1492476
• Replicon: chromosome
• Accession: NZ_CP054523
• Organism: Bifidobacterium longum subsp. infantis strain PI_005

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: HUE64_RS06780
• Protein ID: WP_014484977.1
• Coordinates: 1492120..1492476 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE64_RS06775
• Protein ID: WP_003829235.1
• Coordinates: 1491837..1492133 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE64_RS06750 (HUE64_06755)	1487228..1489906	-	2679	WP_032743694.1	alanine--tRNA ligase	-
HUE64_RS06755 (HUE64_06760)	1490035..1490271	-	237	WP_007053386.1	hypothetical protein	-
HUE64_RS06760 (HUE64_06765)	1490271..1490669	-	399	WP_032743693.1	DUF948 domain-containing protein	-
HUE64_RS06765 (HUE64_06770)	1490752..1491426	-	675	WP_032743692.1	histidine phosphatase family protein	-
HUE64_RS06770 (HUE64_06775)	1491501..1491767	+	267	WP_230581180.1	hypothetical protein	-
HUE64_RS06775 (HUE64_06780)	1491837..1492133	+	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE64_RS06780 (HUE64_06785)	1492120..1492476	+	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE64_RS06785 (HUE64_06790)	1492751..1493353	-	603	WP_032743691.1	transglutaminase family protein	-
HUE64_RS06790 (HUE64_06795)	1493458..1493691	-	234	WP_235625961.1	hypothetical protein	-
HUE64_RS06795 (HUE64_06800)	1493708..1494097	+	390	WP_230581178.1	cytidine deaminase	-
HUE64_RS06800 (HUE64_06805)	1494235..1495017	+	783	WP_032743690.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HUE64_RS06805 (HUE64_06810)	1495196..1495822	-	627	WP_007052130.1	30S ribosomal protein S4	-
HUE64_RS06810 (HUE64_06815)	1496021..1497013	-	993	WP_080714690.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T159830 WP_014484977.1 NZ_CP054523:1492120-1492476 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T159830 NZ_CP070152:3981511-3981813 [Escherichia coli]
ATGGCTCAAAAGAATAATATAAGGAGTTTTCGTGATGCCTGGCTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTGTCTGGGAAATTACAGGAATATTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATATTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT159830 WP_003829235.1 NZ_CP054523:1491837-1492133 [Bifidobacterium longum subsp. infantis]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT159830 NZ_CP070152:3981849-3982190 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTACTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAATAATCGTAAACTCACCACTG
AAATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A0A0L7D489