Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 1003154..1003737 Replicon chromosome
Accession NZ_CP054523
Organism Bifidobacterium longum subsp. infantis strain PI_005

Toxin (Protein)


Gene name PumA Uniprot ID A0A1S2VZ23
Locus tag HUE64_RS04420 Protein ID WP_032745284.1
Coordinates 1003435..1003737 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I3AVV5
Locus tag HUE64_RS04415 Protein ID WP_007057517.1
Coordinates 1003154..1003438 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE64_RS04390 (HUE64_04405) 999298..999666 - 369 WP_155270629.1 hypothetical protein -
HUE64_RS04395 (HUE64_04410) 999660..1000742 + 1083 Protein_851 transposase -
HUE64_RS04400 (HUE64_04415) 1000840..1001748 + 909 WP_032744304.1 ABC transporter ATP-binding protein -
HUE64_RS04405 (HUE64_04420) 1001745..1002476 + 732 WP_032744305.1 ABC transporter permease -
HUE64_RS04410 (HUE64_04425) 1002494..1003045 - 552 Protein_854 IS30 family transposase -
HUE64_RS04415 (HUE64_04430) 1003154..1003438 - 285 WP_007057517.1 putative addiction module antidote protein Antitoxin
HUE64_RS04420 (HUE64_04435) 1003435..1003737 - 303 WP_032745284.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUE64_RS04425 (HUE64_04440) 1003775..1004071 - 297 WP_032745282.1 hypothetical protein -
HUE64_RS04430 (HUE64_04445) 1004230..1004718 + 489 WP_032745281.1 D-aminoacyl-tRNA deacylase -
HUE64_RS04435 (HUE64_04450) 1004879..1007998 + 3120 WP_136527129.1 alpha-mannosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1002494..1002949 455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(9-92)

Antitoxin

Uncharacterized protein HI1420

(3-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11350.08 Da        Isoelectric Point: 10.4626

>T159828 WP_032745284.1 NZ_CP054523:c1003737-1003435 [Bifidobacterium longum subsp. infantis]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Download         Length: 303 bp

>T159828 NZ_CP070152:3757866-3758264 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACAGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10158.37 Da        Isoelectric Point: 4.3707

>AT159828 WP_007057517.1 NZ_CP054523:c1003438-1003154 [Bifidobacterium longum subsp. infantis]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Download         Length: 285 bp

>AT159828 NZ_CP070152:3757570-3757863 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2VZ23

Antitoxin

Source ID Structure
AlphaFold DB I3AVV5

References