Detailed information of TA system TA159819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1361479..1362118
• Replicon: chromosome
• Accession: NZ_CP054521
• Organism: Bifidobacterium longum subsp. infantis strain PI_008

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W6EZW1
• Locus tag: HUE67_RS05825
• Protein ID: WP_014484977.1
• Coordinates: 1361479..1361835 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HUE67_RS05830
• Protein ID: WP_003829235.1
• Coordinates: 1361822..1362118 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUE67_RS05795 (HUE67_05800)	1356942..1357934	+	993	WP_080714690.1	ABC transporter ATP-binding protein	-
HUE67_RS05800 (HUE67_05805)	1358133..1358759	+	627	WP_007052130.1	30S ribosomal protein S4	-
HUE67_RS05805 (HUE67_05810)	1358938..1359720	-	783	WP_032743690.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
HUE67_RS05810 (HUE67_05815)	1359858..1360247	-	390	WP_230581178.1	cytidine deaminase	-
HUE67_RS05815 (HUE67_05820)	1360264..1360497	+	234	WP_235625961.1	hypothetical protein	-
HUE67_RS05820 (HUE67_05825)	1360602..1361204	+	603	WP_032743691.1	transglutaminase family protein	-
HUE67_RS05825 (HUE67_05830)	1361479..1361835	-	357	WP_014484977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HUE67_RS05830 (HUE67_05835)	1361822..1362118	-	297	WP_003829235.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HUE67_RS05835 (HUE67_05840)	1362188..1362454	-	267	WP_230581180.1	hypothetical protein	-
HUE67_RS05840 (HUE67_05845)	1362529..1363203	+	675	WP_032743692.1	histidine phosphatase family protein	-
HUE67_RS05845 (HUE67_05850)	1363286..1363684	+	399	WP_032743693.1	DUF948 domain-containing protein	-
HUE67_RS05850 (HUE67_05855)	1363684..1363920	+	237	WP_007053386.1	hypothetical protein	-
HUE67_RS05855 (HUE67_05860)	1364049..1366727	+	2679	WP_032743694.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-111)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (12-54)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13302.25 Da
• Isoelectric Point: 8.9603

>T159819 WP_014484977.1 NZ_CP054521:c1361835-1361479 [Bifidobacterium longum subsp. infantis]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T159819 NZ_CP070152:c1535792-1535457 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATACGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10997.50 Da
• Isoelectric Point: 4.2896

>AT159819 WP_003829235.1 NZ_CP054521:c1362118-1361822 [Bifidobacterium longum subsp. infantis]
MMAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTP
QIRDGAVILPEGWDDDED

Nucleotide

• Length: 297 bp

>AT159819 NZ_CP070152:c1536040-1535792 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGAAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0L7D489