Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1302476..1303025 Replicon chromosome
Accession NZ_CP054521
Organism Bifidobacterium longum subsp. infantis strain PI_008

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag HUE67_RS05535 Protein ID WP_003829109.1
Coordinates 1302476..1302799 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0M4LW25
Locus tag HUE67_RS05540 Protein ID WP_032743660.1
Coordinates 1302786..1303025 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE67_RS05510 (HUE67_05510) 1297969..1298258 - 290 Protein_1062 IS256 family transposase -
HUE67_RS05515 (HUE67_05515) 1298728..1299993 - 1266 WP_050496214.1 Fic family protein -
HUE67_RS05520 (HUE67_05520) 1300535..1300900 - 366 WP_032743659.1 YccF domain-containing protein -
HUE67_RS05525 (HUE67_05525) 1301138..1301497 + 360 WP_080714682.1 class I SAM-dependent methyltransferase -
HUE67_RS05530 (HUE67_05530) 1301645..1302259 + 615 WP_080714683.1 cupin domain-containing protein -
HUE67_RS05535 (HUE67_05535) 1302476..1302799 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HUE67_RS05540 (HUE67_05540) 1302786..1303025 - 240 WP_032743660.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HUE67_RS05545 (HUE67_05545) 1303205..1304461 + 1257 WP_032743661.1 HipA domain-containing protein -
HUE67_RS05550 1304797..1304907 + 111 WP_230581173.1 XRE family transcriptional regulator -
HUE67_RS05555 (HUE67_05555) 1305091..1306362 - 1272 WP_032743663.1 MFS transporter -
HUE67_RS05560 (HUE67_05560) 1306519..1307181 - 663 WP_032743761.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-54)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T159818 WP_003829109.1 NZ_CP054521:c1302799-1302476 [Bifidobacterium longum subsp. infantis]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T159818 NZ_CP070152:c458237-458134 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9121.16 Da        Isoelectric Point: 4.5881

>AT159818 WP_032743660.1 NZ_CP054521:c1303025-1302786 [Bifidobacterium longum subsp. infantis]
MQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDETR

Download         Length: 240 bp

>AT159818 NZ_CP070152:458096-458367 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M4LW25

References