Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2231676..2232259 Replicon chromosome
Accession NZ_CP054520
Organism Bifidobacterium longum subsp. infantis strain PI_009

Toxin (Protein)


Gene name PumA Uniprot ID A0A1S2VZ23
Locus tag HUE68_RS09645 Protein ID WP_032745284.1
Coordinates 2231957..2232259 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I3AVV5
Locus tag HUE68_RS09640 Protein ID WP_007057517.1
Coordinates 2231676..2231960 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE68_RS09615 (HUE68_09655) 2227820..2228188 - 369 WP_155270629.1 hypothetical protein -
HUE68_RS09620 (HUE68_09660) 2228182..2229264 + 1083 Protein_1872 transposase -
HUE68_RS09625 (HUE68_09665) 2229362..2230270 + 909 WP_032744304.1 ABC transporter ATP-binding protein -
HUE68_RS09630 (HUE68_09670) 2230267..2230998 + 732 WP_032744305.1 ABC transporter permease -
HUE68_RS09635 (HUE68_09675) 2231016..2231567 - 552 Protein_1875 IS30 family transposase -
HUE68_RS09640 (HUE68_09680) 2231676..2231960 - 285 WP_007057517.1 putative addiction module antidote protein Antitoxin
HUE68_RS09645 (HUE68_09685) 2231957..2232259 - 303 WP_032745284.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUE68_RS09650 (HUE68_09690) 2232297..2232626 - 330 WP_257798116.1 DUF1922 domain-containing protein -
HUE68_RS09655 (HUE68_09695) 2232752..2233240 + 489 WP_032745281.1 D-aminoacyl-tRNA deacylase -
HUE68_RS09660 (HUE68_09700) 2233401..2236520 + 3120 WP_136527129.1 alpha-mannosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2231016..2231471 455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(9-92)

Antitoxin

Uncharacterized protein HI1420

(3-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11350.08 Da        Isoelectric Point: 10.4626

>T159814 WP_032745284.1 NZ_CP054520:c2232259-2231957 [Bifidobacterium longum subsp. infantis]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Download         Length: 303 bp

>T159814 NZ_CP070148:4878065-4878376 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10158.37 Da        Isoelectric Point: 4.3707

>AT159814 WP_007057517.1 NZ_CP054520:c2231960-2231676 [Bifidobacterium longum subsp. infantis]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Download         Length: 285 bp

>AT159814 NZ_CP070148:4878377-4878667 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2VZ23

Antitoxin

Source ID Structure
AlphaFold DB I3AVV5

References