Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 522877..523460 Replicon chromosome
Accession NZ_CP054519
Organism Bifidobacterium longum subsp. infantis strain PI_010

Toxin (Protein)


Gene name PumA Uniprot ID A0A1S2VZ23
Locus tag HUE69_RS02215 Protein ID WP_032745284.1
Coordinates 523158..523460 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID I3AVV5
Locus tag HUE69_RS02210 Protein ID WP_007057517.1
Coordinates 522877..523161 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUE69_RS02185 (HUE69_02210) 519021..519389 - 369 WP_155270629.1 hypothetical protein -
HUE69_RS02190 (HUE69_02215) 519383..520465 + 1083 Protein_427 transposase -
HUE69_RS02195 (HUE69_02220) 520563..521471 + 909 WP_032744304.1 ABC transporter ATP-binding protein -
HUE69_RS02200 (HUE69_02225) 521468..522199 + 732 WP_032744305.1 ABC transporter permease -
HUE69_RS02205 (HUE69_02230) 522217..522768 - 552 Protein_430 IS30 family transposase -
HUE69_RS02210 (HUE69_02235) 522877..523161 - 285 WP_007057517.1 putative addiction module antidote protein Antitoxin
HUE69_RS02215 (HUE69_02240) 523158..523460 - 303 WP_032745284.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUE69_RS02220 (HUE69_02245) 523498..523827 - 330 WP_257798116.1 DUF1922 domain-containing protein -
HUE69_RS02225 (HUE69_02250) 523953..524441 + 489 WP_032745281.1 D-aminoacyl-tRNA deacylase -
HUE69_RS02230 (HUE69_02255) 524602..527721 + 3120 WP_032745278.1 alpha-mannosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 522217..522672 455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(9-92)

Antitoxin

Uncharacterized protein HI1420

(3-92)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11350.08 Da        Isoelectric Point: 10.4626

>T159804 WP_032745284.1 NZ_CP054519:c523460-523158 [Bifidobacterium longum subsp. infantis]
IKQSAEYRKWFKKLRDHKAKAAIQARLDACKLAGRPFGDIKPVGGPVSEMRFHTGAGYRVYFAMQGNVLMLLLAGGDKST
QQTDIRQAHDILNDYKEQRQ

Download         Length: 303 bp

>T159804 NZ_CP070148:c2220288-2218966 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10158.37 Da        Isoelectric Point: 4.3707

>AT159804 WP_007057517.1 NZ_CP054519:c523161-522877 [Bifidobacterium longum subsp. infantis]
MSTEFTDYDTSEYLENEQDIIAYLNAIAEYDDPALMQAALGNVAKARGMTQIAKDAGVGRESLYKSLSKDGNPSFQTIAK
VIHALGGRLTIQAA

Download         Length: 285 bp

>AT159804 NZ_CP070148:c2220554-2220288 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAATGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTAACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCAAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1S2VZ23

Antitoxin

Source ID Structure
AlphaFold DB I3AVV5

References