Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2053450..2053959 Replicon chromosome
Accession NZ_CP054482
Organism Macrococcus bohemicus strain 19Msa422

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag HT586_RS10785 Protein ID WP_096078043.1
Coordinates 2053450..2053812 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag HT586_RS10790 Protein ID WP_165958642.1
Coordinates 2053813..2053959 (-) Length 49 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HT586_RS10760 2048526..2050676 - 2151 WP_203545786.1 RNA-binding transcriptional accessory protein -
HT586_RS10765 2050757..2051524 - 768 WP_203545787.1 RNA polymerase sigma factor SigB -
HT586_RS10770 2051535..2051975 - 441 WP_188018607.1 anti-sigma B factor RsbW -
HT586_RS10775 2051977..2052303 - 327 WP_096078045.1 anti-sigma factor antagonist -
HT586_RS10780 2052393..2053400 - 1008 WP_203545788.1 PP2C family protein-serine/threonine phosphatase -
HT586_RS10785 2053450..2053812 - 363 WP_096078043.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HT586_RS10790 2053813..2053959 - 147 WP_165958642.1 hypothetical protein Antitoxin
HT586_RS10795 2054022..2055167 - 1146 WP_203545789.1 alanine racemase -
HT586_RS10800 2055224..2055562 - 339 WP_203545790.1 holo-ACP synthase -
HT586_RS10805 2055555..2056034 - 480 WP_096078040.1 PH domain-containing protein -
HT586_RS10810 2056027..2057532 - 1506 WP_203545791.1 PH domain-containing protein -
HT586_RS10815 2057519..2057998 - 480 WP_203545792.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-109)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13231.30 Da        Isoelectric Point: 7.3168

>T159800 WP_096078043.1 NZ_CP054482:c2053812-2053450 [Macrococcus bohemicus]
MKRGDVYLADLSPVKGSEQGGKRPVVIIQNDIGNKYSPTVIVAAITAKINKSKIPTHVEIGKTNHHLDKDSVILLEQIRT
IDKNRLIEKLTYLPKEKMEEVDAALAISLNLSIDDSETLG

Download         Length: 363 bp

>T159800 NZ_CP070148:1104948-1105250 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 49 a.a.        Molecular weight: 5544.19 Da        Isoelectric Point: 3.7510

>AT159800 WP_165958642.1 NZ_CP054482:c2053959-2053813 [Macrococcus bohemicus]
MSKLLEQQLIEGYQSMAEINLLIASEHYLLECEVYDNVDHMSVQNDGE

Download         Length: 147 bp

>AT159800 NZ_CP070148:1105286-1105627 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References