Detailed information of TA system TA159798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3698359..3698996
• Replicon: chromosome
• Accession: NZ_CP054479
• Organism: Bacillus amyloliquefaciens strain R8-25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HT132_RS18200
• Protein ID: WP_003156187.1
• Coordinates: 3698359..3698709 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HT132_RS18205
• Protein ID: WP_003156188.1
• Coordinates: 3698715..3698996 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT132_RS18160	3693399..3694001	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
HT132_RS18165	3694001..3694789	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
HT132_RS18170	3694755..3695237	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
HT132_RS18175	3695234..3695563	-	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HT132_RS18180	3695627..3696634	-	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
HT132_RS18185	3696646..3697047	-	402	WP_003156178.1	anti-sigma regulatory factor	-
HT132_RS18190	3697050..3697415	-	366	WP_007609584.1	RsbT antagonist protein RsbS	-
HT132_RS18195	3697420..3698241	-	822	WP_003156182.1	STAS domain-containing protein	-
HT132_RS18200	3698359..3698709	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HT132_RS18205	3698715..3698996	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HT132_RS18210	3699116..3700285	-	1170	WP_021495079.1	alanine racemase	-
HT132_RS18215	3700402..3701409	-	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
HT132_RS18220	3701574..3701939	-	366	WP_014304402.1	holo-ACP synthase	-
HT132_RS18225	3702032..3702631	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T159798 WP_003156187.1 NZ_CP054479:c3698709-3698359 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T159798 NZ_CP070148:876317-876715 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT159798 WP_003156188.1 NZ_CP054479:c3698996-3698715 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT159798 NZ_CP070148:876021-876314 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5