Detailed information of TA system TA159797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 2922464..2923381
• Replicon: chromosome
• Accession: NZ_CP054479
• Organism: Bacillus amyloliquefaciens strain R8-25

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: HT132_RS14120
• Protein ID: WP_007407256.1
• Coordinates: 2922464..2923210 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HT132_RS14125
• Protein ID: WP_003154807.1
• Coordinates: 2923211..2923381 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HT132_RS14100	2917858..2918808	-	951	WP_007610844.1	ring-cleaving dioxygenase	-
HT132_RS14105	2919130..2920446	+	1317	WP_007610842.1	amino acid permease	-
HT132_RS14110	2920732..2921349	+	618	WP_003154804.1	DUF47 domain-containing protein	-
HT132_RS14115	2921362..2922360	+	999	WP_003154805.1	inorganic phosphate transporter	-
HT132_RS14120	2922464..2923210	+	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HT132_RS14125	2923211..2923381	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HT132_RS14130	2923638..2924516	-	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
HT132_RS14135	2924530..2924793	-	264	WP_174532596.1	phage holin	-
HT132_RS14140	2924807..2925070	-	264	WP_021493821.1	hemolysin XhlA family protein	-
HT132_RS14145	2925122..2925883	-	762	WP_160216823.1	phage portal protein	-
HT132_RS14150	2925940..2926137	-	198	WP_007610833.1	XkdX family protein	-
HT132_RS14155	2926142..2926513	-	372	WP_017417608.1	hypothetical protein	-
HT132_RS14160	2926526..2928157	-	1632	WP_174532597.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2914139..2957644	43505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T159797 WP_007407256.1 NZ_CP054479:2922464-2923210 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T159797 NZ_CP070148:c607623-607471 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT159797 WP_003154807.1 NZ_CP054479:2923211-2923381 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT159797 NZ_CP070148:607672-607729 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14