Detailed information of TA system TA159786

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2199415..2201099
• Replicon: chromosome
• Accession: NZ_CP054475
• Organism: Thalassolituus hydrocarbonoclasticus strain alknpb1M-1

Toxin (Protein)

• Gene name: HipA3
• Uniprot ID: -
• Locus tag: HUF19_RS09695
• Protein ID: WP_260996464.1
• Coordinates: 2199415..2200743 (-)
• Length: 443 a.a.

Antitoxin (Protein)

• Gene name: HipB3
• Uniprot ID: -
• Locus tag: HUF19_RS09700
• Protein ID: WP_260996465.1
• Coordinates: 2200737..2201099 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUF19_RS09680 (HUF19_09645)	2195544..2197016	+	1473	WP_260996461.1	RimK family protein	-
HUF19_RS09685 (HUF19_09650)	2197266..2198393	+	1128	WP_260996462.1	response regulator	-
HUF19_RS09690 (HUF19_09655)	2198384..2199418	-	1035	WP_260996463.1	DUF3524 domain-containing protein	-
HUF19_RS09695 (HUF19_09660)	2199415..2200743	-	1329	WP_260996464.1	type II toxin-antitoxin system HipA family toxin	Toxin
HUF19_RS09700 (HUF19_09665)	2200737..2201099	-	363	WP_260996465.1	helix-turn-helix transcriptional regulator	Antitoxin
HUF19_RS09705 (HUF19_09670)	2201269..2202612	-	1344	WP_260996466.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HUF19_RS09710 (HUF19_09675)	2202836..2203552	+	717	WP_260996467.1	pseudouridine synthase	-
HUF19_RS09715 (HUF19_09680)	2203739..2206051	+	2313	WP_260996468.1	DUF6351 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (178-414)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 443 a.a.
• Molecular weight: 49167.98 Da
• Isoelectric Point: 7.9655

>T159786 WP_260996464.1 NZ_CP054475:c2200743-2199415 [Thalassolituus hydrocarbonoclasticus]
MVMEAIRVQYAEGAVTHSVGAVSFDTTTGQGAFEYSPEFIRSGIELAPLTMPLAAGVYRFPQLGFATFKGLPGLIADSLP
DDFGNAVLNAWVASRGKRPQGITPLQRLQYTGKRGMGALEFAPATRLKSLNASQQVEIRALVEIAQDILDKRSDFQVTLD
QHGADDKEAMLALLSVGMSAGGARPKAVLAFNDDFTQARSGQTDVPAGFSHYLMKFDGVSEHNQNTETFGDPLGYGAMEY
VYAQMAQACGIQMMPCYLLAEGDRRHFLTRRFDREGNDKIHMQTLNGLAHVDYKKPGSFSYAELFAVARRLKLSAPEAEQ
ILRRMVFNIVARNHDDHAKNTAFLYRNRKWSLAPAYDLAYSYKPGSPWVNNHWMSLNGKRDGFTREDIFSLEKLSPLFSR
KKLNTVIDEAIEQVSRWPQLAKEQGVPLSLVNEITANLRLTL

Nucleotide

• Length: 1329 bp

>T159786 NZ_CP070134:c5091920-5091813 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 12831.50 Da
• Isoelectric Point: 6.3214

>AT159786 WP_260996465.1 NZ_CP054475:c2201099-2200737 [Thalassolituus hydrocarbonoclasticus]
MSSVSSYSDLSPAALSELLGERLKLARLNQDLTQAQVAEQAGVSRKVVINAEKGKASLEAFVAVLQALNQVSQLDQFLPP
QTLSPLQLAKLQGKRRQRASGLGLGQQVTDTGQAEEDGSW

Nucleotide

• Length: 363 bp

>AT159786 NZ_CP070134:5091968-5092034 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCTC