Detailed information of TA system TA159777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3595765..3596986
• Replicon: chromosome
• Accession: NZ_CP054472
• Organism: Pseudomonas aeruginosa strain PAAK088

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: MS088_RS16545
• Protein ID: WP_003102740.1
• Coordinates: 3596408..3596986 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MS088_RS16540
• Protein ID: WP_003088112.1
• Coordinates: 3595765..3596319 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS088_RS16515	3591333..3591884	+	552	WP_003102727.1	XRE family transcriptional regulator	-
MS088_RS16520	3591881..3592279	-	399	WP_023082958.1	NADH-quinone oxidoreductase subunit A	-
MS088_RS16525	3592292..3592615	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
MS088_RS16530	3592946..3593407	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
MS088_RS16535	3593412..3595607	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
MS088_RS16540	3595765..3596319	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
MS088_RS16545	3596408..3596986	+	579	WP_003102740.1	HD domain-containing protein	Toxin
MS088_RS16550	3597095..3598282	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MS088_RS16555	3598272..3600443	-	2172	WP_009876279.1	type I secretion system permease/ATPase	-
MS088_RS16560	3600433..3601711	-	1279	Protein_3277	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T159777 WP_003102740.1 NZ_CP054472:3596408-3596986 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T159777 NZ_CP070134:c3267810-3267433 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT159777 WP_003088112.1 NZ_CP054472:3595765-3596319 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT159777 NZ_CP070134:c3268268-3267900 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07