Detailed information of TA system TA159775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 1889516..1891024
• Replicon: chromosome
• Accession: NZ_CP054472
• Organism: Pseudomonas aeruginosa strain PAAK088

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MS088_RS08775
• Protein ID: WP_012613851.1
• Coordinates: 1889516..1890103 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: MS088_RS08780
• Protein ID: WP_012613852.1
• Coordinates: 1890170..1891024 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS088_RS08770	1885771..1889250	-	3480	WP_023115144.1	hybrid sensor histidine kinase/response regulator	-
MS088_RS08775	1889516..1890103	+	588	WP_012613851.1	GNAT family N-acetyltransferase	Toxin
MS088_RS08780	1890170..1891024	+	855	WP_012613852.1	AraC family transcriptional regulator	Antitoxin
MS088_RS08785	1891121..1893286	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
MS088_RS08790	1893461..1895335	+	1875	WP_003091605.1	MFS transporter	-
MS088_RS08795	1895396..1895605	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T159775 WP_012613851.1 NZ_CP054472:1889516-1890103 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAVLEASFTG

Nucleotide

• Length: 588 bp

>T159775 NZ_CP070134:2972894-2973205 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31959.27 Da
• Isoelectric Point: 7.3276

>AT159775 WP_012613852.1 NZ_CP054472:1890170-1891024 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHADSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLPVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Nucleotide

• Length: 855 bp

>AT159775 NZ_CP070134:2973206-2973496 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG