Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 133901..134550 Replicon chromosome
Accession NZ_CP054470
Organism Burkholderia gladioli strain CGB10

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HUB32_RS36730 Protein ID WP_145757807.1
Coordinates 134140..134550 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag HUB32_RS36725 Protein ID WP_145757811.1
Coordinates 133901..134143 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUB32_RS36710 129034..130008 - 975 WP_013699799.1 RepA replicase -
HUB32_RS36715 131192..132214 - 1023 WP_145757813.1 ParB/RepB/Spo0J family partition protein -
HUB32_RS36720 132217..132870 - 654 WP_036032694.1 AAA family ATPase -
HUB32_RS36725 133901..134143 + 243 WP_145757811.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HUB32_RS36730 134140..134550 + 411 WP_145757807.1 type II toxin-antitoxin system VapC family toxin Toxin
HUB32_RS36735 134966..135103 + 138 WP_186289384.1 hypothetical protein -
HUB32_RS36740 135416..135703 + 288 WP_145757805.1 hypothetical protein -
HUB32_RS36745 136166..136423 + 258 WP_186289385.1 hypothetical protein -
HUB32_RS36750 137109..137534 + 426 WP_186289386.1 DUF2384 domain-containing protein -
HUB32_RS36755 137531..138064 + 534 WP_186289387.1 RES family NAD+ phosphorylase -
HUB32_RS36760 139183..139425 + 243 WP_186289388.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 87124..150257 63133


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-125)

Antitoxin

SpoVT-AbrB domain

(12-48)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14778.17 Da        Isoelectric Point: 6.4515

>T159772 WP_145757807.1 NZ_CP054470:134140-134550 [Burkholderia gladioli]
MTLYLLDTNILSNVMRDPRGACATRIGLTPPEHLCTSIIVAAELRFGVLKRGSSTLAQRVEQLLASLPVLPLQADADRCY
GQLRAELEKQGQLIGANDMLIAAHALAVEAVLVTDNTAEFARVAGLRIENWLRPTT

Download         Length: 411 bp

>T159772 NZ_CP070134:c2547802-2547695 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9250.55 Da        Isoelectric Point: 4.6234

>AT159772 WP_145757811.1 NZ_CP054470:133901-134143 [Burkholderia gladioli]
MPTHDIRHARLFRNGRSQAVRIPREFELAVSEVTIYREGLRLIIEPVPAAPLRELFAQWTPLDETFPDIENLSAEPVDIE

Download         Length: 243 bp

>AT159772 NZ_CP070134:2547850-2547916 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References