Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2780938..2781458 Replicon chromosome
Accession NZ_CP054469
Organism Burkholderia gladioli strain CGB10

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag HUB32_RS31090 Protein ID WP_186229286.1
Coordinates 2781234..2781458 (-) Length 75 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag HUB32_RS31085 Protein ID WP_036032828.1
Coordinates 2780938..2781237 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUB32_RS31060 2776282..2776737 - 456 WP_013689018.1 CesT family type III secretion system chaperone -
HUB32_RS31065 2776858..2777142 - 285 WP_172535078.1 hypothetical protein -
HUB32_RS31070 2777314..2777559 - 246 WP_013689020.1 hypothetical protein -
HUB32_RS31075 2777589..2778995 - 1407 WP_103694061.1 type III secretion protein -
HUB32_RS31080 2779643..2780833 + 1191 WP_186229285.1 MFS transporter -
HUB32_RS31085 2780938..2781237 - 300 WP_036032828.1 putative addiction module antidote protein Antitoxin
HUB32_RS31090 2781234..2781458 - 225 WP_186229286.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HUB32_RS31095 2781747..2783300 + 1554 WP_186229287.1 hypothetical protein -
HUB32_RS31100 2783335..2783790 + 456 WP_160294542.1 NUDIX domain-containing protein -
HUB32_RS31105 2783847..2785271 - 1425 WP_186247682.1 methyltransferase regulatory domain-containing protein -
HUB32_RS31110 2785718..2786209 + 492 WP_186107217.1 MarR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(18-69)

Antitoxin

Cro/C1-type helix-turn-helix domain

(46-92)


Sequences

Toxin        


Download         Length: 75 a.a.        Molecular weight: 8123.30 Da        Isoelectric Point: 9.5807

>T159770 WP_186229286.1 NZ_CP054469:c2781458-2781234 [Burkholderia gladioli]
MTFFGYRIPDNQHACARGDGVSELRIDHGPGYRVYYMKRGRVVVVLLCGGNKDSQGHDIAVAKAIAAQWKGSTI

Download         Length: 225 bp

>T159770 NZ_CP070134:c2032216-2031920 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10596.95 Da        Isoelectric Point: 4.7420

>AT159770 WP_036032828.1 NZ_CP054469:c2781237-2780938 [Burkholderia gladioli]
MKAVKFSRYDTVDYLQTEEEIAAYLDAAVEDGDPALIAAALGDIARARNISQLARDTGMSREGIYRALSGDGNPSFATVM
KIANALGLRISFRPATSSP

Download         Length: 300 bp

>AT159770 NZ_CP070134:c2031918-2031523 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References