Detailed information of TA system TA159768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1143158..1143800
• Replicon: chromosome
• Accession: NZ_CP054469
• Organism: Burkholderia gladioli strain CGB10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2LQ48
• Locus tag: HUB32_RS24050
• Protein ID: WP_013690721.1
• Coordinates: 1143158..1143580 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUB32_RS24055
• Protein ID: WP_123038586.1
• Coordinates: 1143567..1143800 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUB32_RS24030	1138946..1140319	+	1374	WP_186028012.1	L-fucose:H+ symporter permease	-
HUB32_RS24035	1140322..1141404	+	1083	WP_186065501.1	aldose 1-epimerase family protein	-
HUB32_RS24040	1141424..1142194	-	771	WP_017918829.1	DNA-binding transcriptional repressor DeoR	-
HUB32_RS24045	1142356..1143036	+	681	WP_098183150.1	deoxyribose-phosphate aldolase	-
HUB32_RS24050	1143158..1143580	-	423	WP_013690721.1	type II toxin-antitoxin system VapC family toxin	Toxin
HUB32_RS24055	1143567..1143800	-	234	WP_123038586.1	DNA-binding protein	Antitoxin
HUB32_RS24060	1144143..1144550	+	408	WP_186289213.1	hypothetical protein	-
HUB32_RS24065	1144728..1145762	+	1035	WP_160295090.1	flavodoxin family protein	-
HUB32_RS24070	1145978..1147114	+	1137	WP_013690743.1	glutathione-independent formaldehyde dehydrogenase	-
HUB32_RS24075	1147111..1147437	-	327	WP_186103268.1	DUF1328 domain-containing protein	-
HUB32_RS24080	1147430..1147657	-	228	WP_105853279.1	hypothetical protein	-
HUB32_RS24085	1147879..1148196	-	318	WP_124083530.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15649.91 Da
• Isoelectric Point: 8.5438

>T159768 WP_013690721.1 NZ_CP054469:c1143580-1143158 [Burkholderia gladioli]
MYLIDTNVVSEVRKRDRADKGVMAFFRQAARDDAGLYLSVVTVGELRRGVEIIRHRGDESQATRLANWLDGVLREFAANI
LVVDKEIGQMWGYLRVPRSEHSLDKVIAATALIHDLTVVTRNVDDFAGTGARVLNPFKGP

Nucleotide

• Length: 423 bp

>T159768 NZ_CP070134:c1828370-1827963 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.65 Da
• Isoelectric Point: 5.0618

>AT159768 WP_123038586.1 NZ_CP054469:c1143800-1143567 [Burkholderia gladioli]
MANLLVRNVDEMLVQILRERAAAHGHSAEAEHREILARALREPQRKTFAQVLMGMPNVGSDADFARVDDGEAANVFD

Nucleotide

• Length: 234 bp

>AT159768 NZ_CP070134:c1828617-1828351 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A833Q1H0