Detailed information of TA system TA159766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3980205..3981481
• Replicon: chromosome
• Accession: NZ_CP054468
• Organism: Burkholderia gladioli strain CGB10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HUB32_RS17905
• Protein ID: WP_186050498.1
• Coordinates: 3980897..3981481 (+)
• Length: 195 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HUB32_RS17900
• Protein ID: WP_103690443.1
• Coordinates: 3980205..3980795 (+)
• Length: 197 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUB32_RS17880	3976799..3977236	+	438	WP_080742279.1	DUF4212 domain-containing protein	-
HUB32_RS17885	3977230..3979245	+	2016	WP_036049934.1	VC_2705 family sodium/solute symporter	-
HUB32_RS17890	3979289..3979723	+	435	WP_126242584.1	hypothetical protein	-
HUB32_RS17895	3979790..3980053	+	264	WP_013698802.1	PAAR domain-containing protein	-
HUB32_RS17900	3980205..3980795	+	591	WP_103690443.1	helix-turn-helix domain-containing protein	Antitoxin
HUB32_RS17905	3980897..3981481	+	585	WP_186050498.1	HD domain-containing protein	Toxin
HUB32_RS17915	3981976..3982185	+	210	WP_186038650.1	hypothetical protein	-
HUB32_RS17920	3982373..3983110	+	738	WP_046582311.1	hypothetical protein	-
HUB32_RS17925	3983515..3984327	+	813	WP_186014624.1	transglycosylase SLT domain-containing protein	-
HUB32_RS17930	3984522..3985484	+	963	WP_046582898.1	AraC family transcriptional regulator	-
HUB32_RS17935	3985609..3986025	-	417	WP_186046350.1	DUF302 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-171)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-73)

Sequences

Toxin

Protein

• Length: 195 a.a.
• Molecular weight: 21527.27 Da
• Isoelectric Point: 5.0097

>T159766 WP_186050498.1 NZ_CP054468:3980897-3981481 [Burkholderia gladioli]
MDDALIDARLDFLRGCERLKDVLRSGHTSGGRRESTAEHSWRLALMAMVLMDQLGEVDPGRVLELCLVHDLGEALGGDVP
APEQAPGSDKDAVERRDLLRLGDALDAPMRERIVALWDEYARAATPEARAVKALDKIETILQHTQGENPPDFDYAFNLSY
GQAHTRKVPALQRFRERVDEATRERMARAGGPSG

Nucleotide

• Length: 585 bp

>T159766 NZ_CP070134:c1598503-1598192 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTATACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 197 a.a.
• Molecular weight: 21482.35 Da
• Isoelectric Point: 8.7083

>AT159766 WP_103690443.1 NZ_CP054468:3980205-3980795 [Burkholderia gladioli]
MKPISSNQATTTSLPPSALACSPGASLRRWRTLRRIKQEHAAALFGVSQSTISRWEAGTQAVSHEELRRIEACLSARLDS
AADRALERLVSHHTRPVHLVCDLTHRLLACSAPRAAEFAAPLSSLMGASLWPFATAEIVAREQSLRDAGWHDHPAPSIEF
DTGSNDSPQVPIRASRCRWTRFTLSDGTLARLVETL

Nucleotide

• Length: 591 bp

>AT159766 NZ_CP070134:c1598195-1597776 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCCGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTACGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGTGTTA
AGCCGGAATGGTTTCTTTGA